In situ activated T lymphocytes in active versus stable periodontal lesions.
In situ activated T lymphocytes in active versus stable periodontal lesions.
复制标题
活动性牙周病变与稳定性牙周病变中原位激活的 T 淋巴细胞。
DOI：
10.1111/j.1600-0765.1988.tb01420.x
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发表时间：
1988
影响因子：
3.5
通讯作者：
Kaldahl,WB
中科院分区：
文献类型：
--
作者：
Reinhardt,RA;McDonald,TL;Bolton,RW;DuBois,LM;Feely,DE;Kaldahl,WB
建立了一种双标记技术来检测牙周活检点(过去3个月内≥mm附着丧失)、临床相似但稳定或健康的牙周活检部位浸润性细胞上的T细胞表型和HLADR(活化)抗原。从13例牙周维持性疾病患者的上述疾病类别的活检组织中获得连续冷冻切片，并用Leu系列(T细胞亚群)亲和素-过氧化物酶和抗人类白细胞抗原-DR间接碱性磷酸酶标记法进行处理。在每个切片的脑沟、中部和口腔的1/3处重复计数标记细胞，并计算激活的人类白细胞抗原-DR+T细胞。HLADR标记的细胞总数、HLADR阳性的PAN-T细胞和辅助性T细胞(Th)在活动性标本中均多于健康部位(p<；0.05)，在脑沟部位均多于口腔1/3(p<；0.05)。尽管激活的T抑制细胞(T5)密度不随组织位置的不同而有统计学差异，但活动性活检的每视野细胞数比稳定标本或健康标本多(p<；0.05)，尤其是在脑沟。在活动、稳定和健康的活检组织中，大多数PAN-T、T和T细胞似乎显示出激活标记。本研究支持T淋巴细胞在调节牙周组织免疫反应中的功能作用。
A double labeling technique was developed to detect T cell phenotype and HLA‐DR (activation) antigens on infiltrating cells in gingival biopsy sites classified as either periodontally active (≥ mm attachment loss within past 3 months), clinically similar but stable, or healthy. Serial cryostat sections were obtained from each of the above disease category biopsies in 13 periodontal maintenance patients, and were processed with sequential Leu series (T cell subsets) avidinbiotin‐peroxidase followed by anti‐HLA‐DR indirect alkaline phosphatase labeling protocols. Labeled cells were counted in repeatable fields in the sulcular, middle and oral thirds of each section, and HLA‐DR+T cells (activated) were calculated. Total HLA‐DR‐labeled cells and HLA‐DR‐positive pan‐T cells and T helper cells (Th) were all more numerous in active specimens than in healthy sites (p<0.05), and in sulcular fields than in the oral third of the section (p<0.05). Although activated T suppressor cell (T5) densities did not vary statistically according to tissue location, active biopsies had more cells per field than either stable or healthy specimens (p<0.05), especially in the sulcular third. A majority of pan‐T, Tsand Thcells appeared to display activation markers in active, stable and healthy biopsies. This study supports the functional role of T lymphocytes in modulating the immune response in periodontal tissues.