INFLUENCE OF STERILIZATION METHODS ON SELECTED SOIL MICROBIOLOGICAL, PHYSICAL, AND CHEMICAL-PROPERTIES
INFLUENCE OF STERILIZATION METHODS ON SELECTED SOIL MICROBIOLOGICAL, PHYSICAL, AND CHEMICAL-PROPERTIES
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DOI：
10.2134/jeq1989.00472425001800010007x
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发表时间：
1989-01-01
影响因子：
2.4
通讯作者：
LAVY, TL
中科院分区：
文献类型：
--
作者：
WOLF, DC;DAO, TH;LAVY, TL
只有在微生物对不稳定有机化合物的降解受到抑制的情况下，才能从土壤中吸附不稳定有机化合物的研究中获得有效的结果。研究了干热、钴-60辐照、环氧丙烷、氯化汞、高压蒸汽灭菌、叠氮化钠、微波、氯仿和抗生素对三种粉质壤土的杀菌效果。在空气干燥后开始这些处理，将过筛的土壤润湿至-30kPa水势并在25 ℃下培养48小时。 C.灭菌是有效的，并且通过钴-60辐射、环氧丙烷、氯化汞和高压灭菌2次来消除微生物。或3倍。如在马铃薯-葡萄糖琼脂、平板计数琼脂和营养肉汤上缺乏微生物生长所证明的。大多数灭菌处理改变了土壤理化性质。环氧丙烷显着减少了三种土壤的测量表面积。环氧丙烷和叠氮化钠产生的平均pH值增加0.7和0.3个单位，分别为三种土壤。除氯化汞外，所有有效的土壤改良方法均显著提高了三种土壤中锰的提取量。没有有效的处理显着影响阳离子交换容量或水平或可提取的钙，镁，钾的土壤。氯化汞可有效杀菌，对土壤化学和物理性质的影响极小。在采取适当的保障措施后，氯化汞可用于防止在评价土壤对不稳定有机化学品吸附的研究中发生微生物降解。
Valid results from studies of adsorption of labile organic compounds in soil can be obtained only if microbial degradation of the compound is inhibited. This study investigated effectiveness of sterilization of three silt loams with dry heat, cobalt-60 irradiation, propylene oxide, mercuric chloride, autoclaving, sodium azide, microwave, chloroform, and antibiotics. These treatments were begun after air-dried, sieved soil was moistened to -30 kPa water potential and incubated for 48 h at 25.degree. C. Sterilization was effective and micro-organisms were eliminated by cobalt-60 irradiation, propylene oxide, mercuric chloride, and autoclaving 2 .times. or 3 .times. as evidenced by a lack of microbial growth on potato-glucose agar, plate count agar, and nutrient broth. Soil physical and chemical properties were altered by most sterilization treatments. Measured surface areas of the three soils were significantly reduced by propylene oxide. Propylene oxide and sodium azide produced an average pH increase for the three soils of 0.7 and 0.3 units, respectively. All effective steerilization methods except mercuric chloride significantly increased extractable Mn levels in the three soils. None of the effective treatments significantly influenced cation exchange capacity or levels or extractable Ca, Mg, and K of the soils. Mercuric chloride results in effective sterilization with minimal changes in soil chemical and physical properties. Following proper safeguards, mercuric chloride could be used to prevent microbial degradation in studies to evaluate adsorption of labile organic chemicals by soil.