TRPV4 is endogenously expressed in vertebrate spermatozoa and regulates intracellular calcium in human sperm
TRPV4 is endogenously expressed in vertebrate spermatozoa and regulates intracellular calcium in human sperm
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DOI：
10.1016/j.bbrc.2016.03.071
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发表时间：
2016-05-13
影响因子：
3.1
通讯作者：
Goswami, Chandan
中科院分区：
文献类型：
--
作者：
Kumar, Ashutosh;Majhi, Rakesh Kumar;Goswami, Chandan
瞬时受体电位香草蛋白亚型4 （TRPV4）是一种非选择性阳离子通道，参与调节温度、渗透压和不同配体依赖性Ca2+内流。最近，我们已经证明TRPV4在所有脊椎动物中都是保守的。现在我们证明了TRPV4在从鱼类到哺乳动物的所有脊椎动物精子细胞中都是内源性表达的。在人类精子中，TRPV4以n -糖基化蛋白的形式存在，其激活可诱导Ca2+内流。它在游向和游向细胞中的表达和定位不同，表明TRPV4是精子运动的重要决定因素。我们证明了TRPV4的药理激活或抑制调节Ca2+波从头部到尾部的传播。这一发现在男性生育、不育、避孕和濒危物种保护等方面具有广泛的应用价值。(C) 2016 Elsevier Inc.版权所有。
Transient Receptor Potential Vanilloid sub-type 4 (TRPV4) is a non-selective cationic channel involved in regulation of temperature, osmolality and different ligand-dependent Ca2+-influx. Recently, we have demonstrated that TRPV4 is conserved in all vertebrates. Now we demonstrate that TRPV4 is endogenously expressed in all vertebrate sperm cells ranging from fish to mammals. In human sperm, TRPV4 is present as N-glycosylated protein and its activation induces Ca2+-influx. Its expression and localization differs in swim-up and swim-down cells suggesting that TRPV4 is an important determining factor for sperm motility. We demonstrate that pharmacological activation or inhibition of TRPV4 regulates Ca2+-wave propagation from head to tail. Such findings may have wide application in male fertility infertility, contraception and conservation of endangered species as well. (C) 2016 Elsevier Inc. All rights reserved.