Influence of sample return time and ambient temperature on the performance of an immunochemical faecal occult blood test with a new buffer for colorectal cancer screening
Influence of sample return time and ambient temperature on the performance of an immunochemical faecal occult blood test with a new buffer for colorectal cancer screening
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DOI：
10.1097/cej.0000000000000153
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发表时间：
2016-03-01
影响因子：
2.4
通讯作者：
Lepage, Come
中科院分区：
文献类型：
--
作者：
Dancourt, Vincent;Hamza, Samia;Lepage, Come
通过粪便免疫化学试验（FIT）测得的血红蛋白浓度可能会在样本返回延迟或高温的情况下降低。这是一个非常重要的问题。本研究的目的是调查样品返回时间和季节的FIT（FOB黄金）与新的缓冲性能的影响。该研究包括20371名参与法国组织的结直肠癌（CRC）筛查计划的参与者。根据样本返回时间和筛查季节分析阳性筛查试验的概率、CRC和晚期腺瘤的检出率和阳性预测值。将阳性FIT样品在20 ℃或30 ℃的培养箱中储存7天。样本返回时间为3天的阳性率为4.1%，4-5天为4.1%，6-7天为4.6%（P=0.25）。在多因素分析中，CRC和晚期腺瘤的阳性率、检出率和阳性预测值与样本返回时间或筛查季节无关。在20摄氏度的恒温下，7天后血红蛋白浓度降低了5.1%。在30摄氏度的温度下，下降幅度达到20.5%。在培养箱中储存的前4天，其仅为4.5%。使用新的缓冲液，样品返回或季节延迟不会影响临床结果。当温度达到30摄氏度时，粪便样本必须立即返回。
The haemoglobin concentration measured by faecal immunochemical tests (FIT) may be decreased in cases of delayed sample return or high temperature. It is an issue of great importance. The aim of this study was to investigate the effects of sample return time and of season on the performance of an FIT (FOB-Gold) with a new buffer. The study included 20371 participants involved in the French organized colorectal cancer (CRC) screening programme. The probability of a positive screening test, detection rates and positive predictive values for CRC and advanced adenoma were analysed according to sample return time and season of screening. A sample of positive FIT was stored for 7 days in an incubator at 20 degrees C or 30 degrees C. The positivity rate was 4.1% for a sample return time of up to 3 days, 4.1% for 4-5 days and 4.6% for 6-7 days (P=0.25). In multivariate analysis, there was no association between positivity rates, detection rates and positive predictive values for CRC and advanced adenoma and the sample return time or the season of screening. At a constant temperature of 20 degrees C, there was a decrease in the haemoglobin concentration of 5.1% after 7 days. The decrease reached 20.5% at a temperature of 30 degrees C. It was only 4.5% during the first 4 days of storage in the incubator. With the new buffer, delay in sample return or season did not affect the clinical outcome. When temperatures reach 30 degrees C, the faecal sample must be returned promptly.