Induction of increase in intracellular calcium concentration of embryonic cells and acceleration of morphogenetic cell movements during amphibian gastrulation by a 50-Hz magnetic field
Induction of increase in intracellular calcium concentration of embryonic cells and acceleration of morphogenetic cell movements during amphibian gastrulation by a 50-Hz magnetic field
复制标题
DOI：
10.1002/jez.a.359
复制
发表时间：
2007-03-01
影响因子：
2.8
通讯作者：
Takano, Kazuhiro
中科院分区：
文献类型：
--
作者：
Komazaki, Shinji;Takano, Kazuhiro
研究了交变电磁场对两栖类胚胎早期发育的影响。当胚胎在低频电磁场（50 Hz，5-30 mT）的影响下发育时，早期发育速率加快。电磁场的作用优先发生在原肠期，缩短了原肠期。组织学观察表明，电磁场促进原肠胚形成过程中的形态发生细胞运动。使用Fura-2（细胞内钙离子浓度的指示剂）分析在EMF影响下胚胎细胞中的细胞内游离Ca 2+（[Ca 2 +]（i））的浓度。分析了电磁场对囊胚、原肠胚和神经胚细胞[Ca ~（2+）]（i）的影响，电磁场使囊胚、原肠胚和神经胚细胞[Ca ~（2+）]（i）增加，特别是原肠胚细胞。我们的研究结果表明，电磁场专门增加[Ca 2 +]（i）的原肠胚细胞，从而加快在原肠胚形成过程中的形态发生细胞运动的速度。
The influence of an alternating electromagnetic field (EMF) on early development of amphibian embryos was examined. When the embryos developed under the influence of a lowfrequency EMF (50 Hz, 5-30 mT), the rate of early development was accelerated. The effect of EMF was exerted preferentially at the gastrula stage, and the period of gastrulation was shortened. Histological observations showed that EMF promoted morphogenetic cell movements during the gastrulation. The concentration of intracellular free Ca2+ ([Ca2+](i)) in the embryonic cells under the influence of EMF was analyzed using Fura-2, an indicator of the intracellular concentration of calcium ions. The influence of EMF on [Ca2+](i) was analyzed in embryonic cells isolated from blastula, gastrula, and neurula, EMF increased a [Ca2+](i) particularly in the cells isolated from gastrula. Our results suggest that EMF specifically increased the [Ca2+](i) of gastrula cells, thereby, accelerating the rate of morphogenetic cell movements during gastrulation.