Velocity measurement of particulate flow in microfluidic channels using single point confocal fluorescence detection
Velocity measurement of particulate flow in microfluidic channels using single point confocal fluorescence detection
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DOI：
10.1039/b106559a
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发表时间：
2001-01-01
期刊：
影响因子：
4.2
通讯作者：
de Mello, AJ
中科院分区：
文献类型：
--
作者：
Edel, JB;Hill, EK;de Mello, AJ
本文提出了一种非侵入性的光学技术，用于测量微流控通道内的颗粒流动。共聚焦荧光检测用于探测单个荧光标记的微球(直径0.93微米)在不同的流速(50nL min(-1)-8-min(-1))下通过聚焦的激光束。随后使用简单的统计方法来研究所产生的荧光爆发并产生流动粒子的速度数据。流体操纵是通过在聚二甲基硅氧烷(PDMS)衬底中构建的微通道(150微米宽、50微米深)以流体动力泵送流体来实现的。平均荧光爆发频率与流速成正比。此外，恢复的自相关曲线的泊松重现时间和宽度与流速成反比。基于组件的共聚焦荧光检测系统结构简单，可应用于多种平面芯片系统。此外，与常规的多点测量不同，速度测量只涉及沿水流的单个点处的流体系统的询问。
This article presents a non-invasive, optical technique for measuring particulate flow within microfluidic channels. Confocal fluorescence detection is used to probe single fluorescently labeled microspheres (0.93 mum diameter) passing through a focused laser beam at a variety of flow rates (50 nL min(-1)-8 muL min(-1)). Simple statistical methods are subsequently used to investigate the resulting fluorescence bursts and generate velocity data for the flowing particles. Fluid manipulation is achieved by hydrodynamically pumping fluid through microchannels (150 mum wide and 50 mum deep) structured in a polydimethylsiloxane (PDMS) substrate. The mean fluorescence burst frequency is shown to be directly proportional to flow speed. Furthermore, the Poisson recurrence time and width of recovered autocorrelation curves is demonstrated to be inversely proportional to flow speed. The component-based confocal fluorescence detection system is simple and can be applied to a diversity of planar chip systems. In addition, velocity measurement only involves interrogation of the fluidic system at a single point along the flow stream, as opposed to more normal multiple-point measurements.