Esophageal mucin: An adherent mucus gel barrier is absent in the normal esophagus but present in columnar-lined Barrett's esophagus
Esophageal mucin: An adherent mucus gel barrier is absent in the normal esophagus but present in columnar-lined Barrett's esophagus
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DOI：
10.1111/j.1572-0241.2001.04159.x
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发表时间：
2001-09-01
影响因子：
9.8
通讯作者：
Pearson, JP
中科院分区：
文献类型：
--
作者：
Dixon, J;Strugala, V;Pearson, JP
预防措施：在健康食管中是否存在防止胃反流的保护性粘附粘液凝胶屏障尚不确定。其目的是表征表面粘蛋白的组合物，并确定任何粘附的粘液凝胶层的程度上的正常食管，并比较这与Barrett's esophageal.METHODS：分离的表面粘蛋白，其特征在于密度离心，凝胶过滤色谱和化学组成。粘附表面粘液原位可视化的粘膜和活检的未固定和冷冻切片使用的方法，保留粘液层thickness.RESULTS：有一个完全没有粘附的粘液凝胶层上正常的人，猪，和大鼠食管。这与在相应胃粘膜上观察到的厚粘附性粘膜层（中值厚度= 100-200 μ m）形成对比。从猪食道表面分离出少量具有分泌粘蛋白组成特征的糖蛋白。粘蛋白的密度范围在1.44和1.48 g.ml（-1）之间，含有80%的碳水化合物，并且富含丝氨酸、苏氨酸和脯氨酸。粘蛋白在蛋白水解和部分还原时断裂成较小的糖蛋白单位。柱状食管的冷冻切片。活组织检查有大量粘附的表面粘膜层（中位厚度= 90 μ m，四分位距= 84-94 μ m）中性粘蛋白染色（胃型上皮）和酸性粘蛋白结论：一种分泌性粘蛋白，分析结果与胃或唾液粘蛋白不同，在食管粘膜上存在的量非常小，并且其量不足以形成粘附的凝胶层。粘液不太可能在保护正常食管免受反流方面发挥作用。然而，在柱状排列的食管上观察到粘附的粘膜层，这可以防止反流。(Am胃肠病学杂志2001;96：2575-2583. (C)2001年，我胃肠病学）。
OBJECTIVES: The presence of a protective adherent mucus gel barrier against gastric reflux in the healthy esophagus is uncertain. The aim was to characterize the surface mucin composition and determine the extent of any adherent mucus gel layer on the normal esophagus, and compare this with that in Barrett's esophagus.METHODS: Isolated surface mucins were characterized by density centrifugation, gel filtration chromatography and chemical composition. Adherent surface mucus was visualized in situ on unfixed and cryostat sections of mucosa and biopsies using a method that preserves mucus layer thickness.RESULTS: There was a complete absence of adherent mucus gel layers on normal human, pig, and rat esophagi. This was in contrast to the thick adherent mucous layer (median thickness = 100-200 mum) seen on the corresponding gastric mucosa. Small quantities of glycoprotein with a composition characteristic of a secretory mucin were isolated from the pig esophagus surface. The mucin, density range between 1.44 and 1.48 g.ml(-1), contained 80% carbohydrate and was rich in serine, threonine, and proline. The mucin fragmented into smaller glycoprotein units on proteolysis and partially on reduction. Cryostat sections from columnar-lined esophageal. biopsies had a substantial adherent surface mucous layer (median thickness = 90 mum, interquartile range = 84-94 mum) staining for neutral mucins (gastric-type epithelium) and acidic mucins (intestinal metaplasia).CONCLUSIONS: A secretory mucin, with an analysis distinct from that of gastric or salivary mucin, is present in very small quantities on the esophageal mucosa and in amounts insufficient to form an adherent gel layer. It is unlikely that mucus has a role in protecting the normal esophagus against reflux. However, an adherent mucous layer was observed over columnar-lined esophagus, and this may protect against reflux. (Am J Gastroenterol 2001;96:2575-2583. (C) 2001 by Am. Coll. of Gastroenterology).