Effect of Resveratrol on biofilm formation and virulence factor gene expression of Porphyromonas gingivalis in periodontal disease
Effect of Resveratrol on biofilm formation and virulence factor gene expression of Porphyromonas gingivalis in periodontal disease
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DOI：
10.1111/apm.12930
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发表时间：
2019-04-01
期刊：
影响因子：
2.8
通讯作者：
Diwan, Prakash V.
中科院分区：
文献类型：
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作者：
Kugaji, Manohar S.;Kumbar, Vijay M.;Diwan, Prakash V.
牙周病是一种口腔炎症性疾病，会破坏牙齿支持牙周组织，导致牙齿脱落。牙龈卟啉单胞菌是一种关键病原体，在牙周炎中起着重要作用。在之前的研究中，白藜芦醇通过针对炎症和粘附标记物显示出显着的效果。牙龈卟啉单胞菌的毒力因子在细菌粘附和定植中发挥重要作用。在本研究中，我们旨在证明白藜芦醇的抗生物膜和抗菌活性，并利用逆转录聚合酶链反应（RT-PCR）研究白藜芦醇对牙龈卟啉单胞菌毒力因子基因表达的影响。分别通过肉汤微量稀释法和生物膜粘附减少-结晶紫测定法测定白藜芦醇对牙龈卟啉单胞菌的抗菌和抗生物膜活性。我们通过 RT-PCR 对牙龈卟啉单胞菌处理的化合物进行基因表达分析，以分析毒力因子：菌毛和牙龈蛋白酶表达的变化。白藜芦醇对牙龈卟啉单胞菌和其他临床菌株的最低抑菌浓度（MIC）在78.12-156.25μg/mL范围内。白藜芦醇剂量依赖性地阻止生物膜形成，并通过减少菌毛（II 型和 IV 型）和蛋白酶（kgp 和 rgpA）等毒力因子基因的表达来减弱牙龈卟啉单胞菌的毒力。白藜芦醇对牙龈卟啉单胞菌具有卓越的抗菌和抗生物膜活性。用白藜芦醇处理的化合物显着降低了菌毛和牙龈蛋白酶的表达。结果表明，白藜芦醇由于其多种作用，可能成为治疗牙周病的一种简单且廉价的治疗策略。
Periodontal disease is an oral inflammatory disease that destroys the tooth supporting periodontal tissues resulting in tooth loss. Porphyromonas gingivalis is a keystone pathogen that plays a significant role in periodontitis. In previous studies, resveratrol has shown significant results by targeting inflammatory and adhesive markers. Virulence factors of P. gingivalis play an important role in the bacterial adhesion and colonization. In this study, we aimed to demonstrate the anti-biofilm and anti-bacterial activity of resveratrol and also study the effect of resveratrol on the expression of virulence factor genes of P. gingivalis using reverse transcriptase polymerase chain reaction (RT-PCR). The anti-microbial and anti-biofilm activity of resveratrol on P. gingivalis was carried out by broth microdilution assay and biofilm adhesion reduction-crystal violet assay, respectively. We carried out the gene expression analysis by RT-PCR with the P. gingivalis treated compound to analyze the change in the expression of virulence factors: fimbriae and gingipain. Minimal inhibitory concentrations (MIC) of resveratrol against P. gingivalis and other clinical strains are in the range of 78.12-156.25 mu g/mL. Resveratrol dose-dependently prevented the biofilm formation and also attenuated the virulence of P. gingivalis by reducing the expression of virulence factor genes such as fimbriae (type II and IV) and proteinases (kgp and rgpA). Resveratrol demonstrated superior anti-bacterial and anti-biofilm activity against P. gingivalis. There was significant reduction in the expression of fimbriae and gingipain with the resveratrol-treated compound. The results suggest that resveratrol, due to its multiple actions, may become a simple and inexpensive therapeutic strategy for treating periodontal disease.