Spray-dried animal plasma prevents the effects of Staphylococcus aureus enterotoxin B on intestinal barrier function in weaned rats
Spray-dried animal plasma prevents the effects of Staphylococcus aureus enterotoxin B on intestinal barrier function in weaned rats
复制标题
DOI：
10.1093/jn/136.11.2838
复制
发表时间：
2006-11-01
影响因子：
4.2
通讯作者：
Moreto, Miquel
中科院分区：
文献类型：
--
作者：
Perez-Bosque, Anna;Amat, Concepcio;Moreto, Miquel
在这项研究中，我们研究了炎症过程中的肠道屏障功能，以及膳食补充猪喷雾干燥动物血浆（SDAP蛋白和猪免疫球蛋白浓缩物（IC）的影响。Wistar刘易斯大鼠从第21天（断奶）至第34或35天喂食对照饲料或含SDAP或IC的饲料。在第30天和第33天，大鼠接受腹膜内剂量的金黄色葡萄球菌肠毒素B（SE B; 0.5 mg/kg体重; SE B、SEB-SDAP和SEB-IC组）。SEB使空肠电位差降低60%，短路电流降低70%，肠粘膜Na K ATP酶活性降低（均P < 0.05）。葡聚糖通量（4kDa）和辣根过氧化物酶（40 kDa）的跨肠壁通量也在SEB处理的大鼠中增加（分别为P < 0.01，P = 0.068）。SEB还增加了HRP穿过细胞旁间隙的流量（P < 0.05）。此外，SEB治疗组大鼠的紧密连接蛋白表达减少，如ZO-1（减少10%; P < 0.05）和β-连环蛋白（减少20%; P < 0.05）。日粮中添加SDAP或IC可阻止右旋糖酐（P < 0.05）和HRP（P < 0.05）穿过肠上皮的细胞旁流。补充SDAP也能防止SEB对Na-K-ATP酶活性的影响（P < 0.05）。在我们的SEB诱导的肠道炎症模型中，通过肠粘膜的渗透性增加是由于紧密连接蛋白的表达降低，这种作用可以通过SDAP和IC补充来预防。
In this study, we investigated intestinal barrier function during inflammation as well as the effects of dietary supplementation with porcine spray-dried animal plasma (SDAP proteins and porcine immunoglobulin concentrate (IC). Wistar Lewis rats were fed from d 21 (weaning) until d 34 or 35 either a control diet or a diet containing SDAP or IC. On d 30 and d 33, rats received an intraperitoneal dose of Staphylococcus aureus enterotoxin B (SEB; 0.5 mg/kg body wt; groups SEB, SEB-SDAP, and SEB-IC). SEB reduced the potential difference across the jejunum by 60%, the short-circuit current by 70%, and Na-K-ATPase activity in intestinal mucosa (all P < 0.05). The fluxes of dextran flux (4 kDa) and horseradish peroxidase (HRP, 40 kDa) across the intestinal wall also increased in SEB-treated rats (P < 0.01, P = 0.068, respectively). SEB also increased HRP flux across the paracellular space (P < 0.05). Moreover, SEB-treated rats had a reduced expression of tight junction proteins, such as ZO-1 (10% reduction; P < 0.05) and beta-catenin (20% reduction; P < 0.05). Dietary supplementation with SDAP or IC prevented dextran (P < 0.05) and HRP (P < 0.05) paracellular flux across the intestinal epithelium. SDAP supplementation also prevented SEB effects on Na-K-ATPase activity (P < 0.05). In our model of SEB-induced intestinal inflammation, the increased permeability across the intestinal mucosa was due to the lower expression of tight junction proteins, an effect that can be prevented by both SDAP and IC supplementation.