DETERMINATION OF SULFIDE IN BRAIN-TISSUE BY GAS DIALYSIS ION CHROMATOGRAPHY - POSTMORTEM STUDIES AND 2 CASE-REPORTS
DETERMINATION OF SULFIDE IN BRAIN-TISSUE BY GAS DIALYSIS ION CHROMATOGRAPHY - POSTMORTEM STUDIES AND 2 CASE-REPORTS
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DOI：
10.1093/jat/13.2.105
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发表时间：
1989-03-01
影响因子：
2.5
通讯作者：
DOWLING, G
中科院分区：
文献类型：
--
作者：
GOODWIN, LR;FRANCOM, D;DOWLING, G
本文介绍了测定人脑和大鼠脑中硫化物的分析方法。它采用连续流动气体渗析前处理，离子色谱-电化学检测定量。通过腹腔注射NaHS，可可靠地测定致命性中毒后大鼠脑内硫化物水平。通过快速分析样品，可以证明大鼠和人脑中内源性硫化物水平的存在，以及测量中毒后脑内硫化物水平的升高。在死后的大鼠脑组织中，如果身体被冷藏在4摄氏度，在死后96小时仍然可以可靠地证明硫化物水平升高。C.提出了两个人类吸入硫化氢死亡的案例。所描述的方法能够在这两种情况下测量显著升高的硫化物水平。
An analytical method for the determination of sulfide in human and rat brain is described. It utilizes a continuous flow gas dialysis pretreatment and quantitation by ion chromaography with electrochemical detection. Rat brain sulfide levels were reliably measured after fatal intoxication by intraperitoneal injection of NaHS. By expeditious analysis of samples it was possible to demonstrate the presence of endogenous levels of sulfide in both rat and human brain as well as to measure elevated brain levels of sulfide after intoxication. In postmortem rat brain tissue, elevated sulfide levels could still be reliably demonstrated 96 h after death if the bodies had been refrigerated at 4.degree.C. Two case studies of human hydrogen sulfide inhalation fatalities are presented. The described method was able to measure significantly elevated sulfide levels in both cases.