Complete DNA sequence of the mitochondrial genome of the sea-slug, Aplysia californica:: Conservation of the gene order in Euthyneura
Complete DNA sequence of the mitochondrial genome of the sea-slug, Aplysia californica:: Conservation of the gene order in Euthyneura
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DOI：
10.1016/j.ympev.2005.08.017
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发表时间：
2006-02-01
影响因子：
4.1
通讯作者：
Moroz, LL
中科院分区：
文献类型：
--
作者：
Knudsen, B;Kohn, AB;Moroz, LL
我们已经测序并鉴定了实验生物学中一种重要的模式生物，也是腹足纲(腹足纲，后支目)的代表生物--加州海兔的线粒体全基因组。海兔线粒体基因组位于动物线粒体基因组观察大小的较小端(14,117个碱基，NCBI登录号。NC_005827)。与大多数其他线粒体基因组一样，海兔基因组编码2个核糖体亚基RNA(大小rRNA)、22个tRNA和13个蛋白质亚基(细胞色素c氧化酶亚基1-3、细胞色素b辅酶、ATP合成酶亚基6和8以及NADH脱氢酶亚基1-6和4L)。脊椎动物和肺脏动物的基因序列实际上是相同的，大部分差异是由tRNA易位引起的。相比之下，基础腹足类和其他软体动物类别的代表的基因序列与后足类和肺类有很大的不同。将海兔基因组与所有其他已发表的软体动物线粒体基因组进行比较，并使用串联蛋白比对进行系统发育分析。基于蛋白质序列良好比对区域的基于最大似然法的系统发育分析支持真神经节(既包括肺脏也包括后支)和后支(作为一个更具衍生性的类群)的单系进化。本文所测得的海兔线粒体基因组序列将作为利用该模式生物进行比较和神经生物学研究的重要平台。(C)2005 Elsevier Inc.保留所有权利。
We have sequenced and characterized the complete mitochondrial genome of the sea slug, Aplysia californica, an important model organism in experimental biology and a representative of Anaspidea (Opisthobranchia, Gastropoda). The mitochondrial genome of Aplysia is in the small end of the observed sizes of animal mitochondrial genomes (14,117 bp, NCBI Accession No. NC_005827). The Aplysia genome, like most other mitochondrial genomes, encodes genes for 2 ribosomal subunit RNAs (small and large rRNAs), 22 tRNAs, and 13 protein subunits (cytochrome c oxidase subunits 1-3, cytochrome b apoenzyme, ATP synthase subunits 6 and 8, and NADH dehydrogenase subunits 1-6 and 4L). The gene order is virtually identical between opisthobranchs and pulmonates, with the majority of differences arising from tRNA translocations. In contrast, the gene order from representatives of basal gastropods and other molluscan classes is significantly different from opisthobranchs and pulmonates. The Aplysia genome was compared to all other published molluscan mitochondrial genomes and phylogenetic analyses were carried out using a concatenated protein alignment. Phylogenetic analyses using maximum likelihood based analyses of the well aligned regions of the protein sequences support both monophyly of Euthyneura (a group including both the pulmonates and opisthobranchs) and Opisthobranchia (as a more derived group). The Aplysia mitochondrial genome sequenced here will serve as an important platform in both comparative and neurobiological studies using this model organism. (c) 2005 Elsevier Inc. All rights reserved.