Energy metabolism profile of the effects of amino acid treatment on hepatocytes: Phenylalanine and phenylpyruvate inhibit glycolysis of hepatocytes
Energy metabolism profile of the effects of amino acid treatment on hepatocytes: Phenylalanine and phenylpyruvate inhibit glycolysis of hepatocytes
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氨基酸处理对肝细胞影响的能量代谢概况：苯丙氨酸和苯丙酮酸抑制肝细胞的糖酵解
DOI：
10.1016/j.nut.2020.111042
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发表时间：
2021
期刊：
影响因子：
4.4
通讯作者：
Sato Yusuke
中科院分区：
文献类型：
--
作者：
Suzuki Reiko;Sato Yoriko;Fukaya Misato;Suzuki Daisuke;Yoshizawa Fumiaki;Sato Yusuke
目的氨基酸不仅是蛋白质的组成部分，而且还可以代谢为能量物质或作为信号分子。本研究的目的是分析氨基酸处理（必需氨基酸和丙氨酸）是否影响培养肝细胞的能量代谢（糖酵解、线粒体呼吸）。方法将saml12肝细胞分别用5 mM氨基酸处理1 h，用细胞外通量分析仪测定其能量代谢。结果苯丙氨酸和赖氨酸降低了糖酵解的间接指标细胞外酸化率（ECAR），而异亮氨酸和组氨酸提高了ECAR。氨基酸不影响耗氧率，这是线粒体呼吸的间接指标。糖酵解应激试验表明，当培养基中糖酵解底物浓度足够时，苯丙氨酸处理肝细胞抑制糖酵解。我们还研究了苯丙氨酸转化产生的代谢物对肝细胞糖酵解的影响，发现苯丙酮酸抑制糖酵解，而酪氨酸和苯乙胺不影响糖酵解。结论本研究结果补充了氨基酸处理对培养肝细胞能量代谢影响的基础知识，表明苯丙氨酸和苯丙酮酸抑制糖酵解。
ObjectivesAmino acids are not only the building blocks of proteins, but also can be metabolized to energy substances or function as signaling molecules. The aim of this study was to profile whether amino acid treatment (essential amino acids and alanine) affects the energy metabolism (glycolysis, mitochondrial respiration) of cultured hepatocytes.MethodsAML12 hepatocytes were treated with 5 mM of each amino acid for 1 h and the energy metabolism was then measured by using an extracellular flux analyzer.ResultsThe results showed that phenylalanine and lysine decreased the extracellular acidification rate (ECAR), an indirect indicator of glycolysis, whereas isoleucine and histidine increased the ECAR. Amino acids did not affect the oxygen consumption rate, an indirect indicator of mitochondrial respiration. The glycolysis stress test revealed that treatment of the hepatocytes with phenylalanine inhibited glycolysis when the concentration of the substrate for glycolysis was sufficient in cultured media. We also investigated the effect of metabolites derived from conversion of phenylalanine on glycolysis in hepatocytes and found that phenylpyruvate inhibited glycolysis, whereas tyrosine and phenylethylamine did not affect glycolysis.ConclusionsThe findings from the present study complement basic knowledge of the effects of amino acid treatment on energy metabolism in cultured hepatocytes and indicate that phenylalanine and phenylpyruvate inhibit glycolysis.