INTEGRATION AND STABLE GERM LINE TRANSMISSION OF GENES INJECTED INTO MOUSE PRONUCLEI
INTEGRATION AND STABLE GERM LINE TRANSMISSION OF GENES INJECTED INTO MOUSE PRONUCLEI
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DOI：
10.1126/science.6272397
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发表时间：
1981-01-01
期刊：
影响因子：
56.9
通讯作者：
RUDDLE, FH
中科院分区：
文献类型：
--
作者：
GORDON, JW;RUDDLE, FH
通过将遗传物质注入受精卵的原核，成功地将该物质转移到新生小鼠的基因组中。有两种模式的处理注入的DNA：一种是材料不整合到宿主基因组中，另一种是注入的基因与高分子量DNA相关。这些模式通过进一步发展到成年而得以维持。注射的DNA与宿主序列的共价结合，以及这种连接的序列以孟德尔分布传递给后代的2个连续世代。
Genetic material was successfully transferred into the genomes of newborn mice by injection of that material into pronuclei of fertilized eggs. There were 2 patterns of processing the injected DNA: one in which the material was not integrated into the host genome, and another in which the injected genes became associated with high MW DNA. These patterns are maintained through further development to adulthood. The covalent association of injected DNA with host sequences, and transmission of such linked sequences in a Mendelian distribution to 2 succeeding generations of progeny are indicated.