T-GAMMA-DELTA-CELLS IN JUVENILE RHEUMATOID-ARTHRITIS AND RHEUMATOID-ARTHRITIS - IN THE JUVENILE RHEUMATOID-ARTHRITIS SYNOVIUM THE T-GAMMA-DELTA-CELLS EXPRESS ACTIVATION ANTIGENS AND ARE PREDOMINANTLY V-DELTA-1+, AND A SIGNIFICANT PROPORTION OF THESE PATIENTS HAVE ELEVATED PERCENTAGES OF T-GAMMA-DELTA-CELLS
T-GAMMA-DELTA-CELLS IN JUVENILE RHEUMATOID-ARTHRITIS AND RHEUMATOID-ARTHRITIS - IN THE JUVENILE RHEUMATOID-ARTHRITIS SYNOVIUM THE T-GAMMA-DELTA-CELLS EXPRESS ACTIVATION ANTIGENS AND ARE PREDOMINANTLY V-DELTA-1+, AND A SIGNIFICANT PROPORTION OF THESE PATIENTS HAVE ELEVATED PERCENTAGES OF T-GAMMA-DELTA-CELLS
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DOI：
10.1111/j.1365-3083.1990.tb03207.x
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发表时间：
1990-12-01
影响因子：
3.7
通讯作者：
NATVIG, JB
中科院分区：
文献类型：
--
作者：
KJELDSENKRAGH, J;QUAYLE, A;NATVIG, JB
用抗TcR-γ/β-1单抗和流式细胞仪检测γ-γ/β-1的数量。从24例幼年类风湿性关节炎(JRA)、5例成人类风湿性关节炎(JRA)和14例类风湿关节炎(RA)患者的外周血和滑液或组织的配对样本中提取细胞。滑膜室T、Gamma、……增量无明显差异。与JRA、成年JRA或RA患者的血液进行比较。在外周血或滑膜室中也没有发现任何显著的差异。与正常对照组的外周血进行比较。然而，7名患有JRA的儿童具有非常高的T.Gamma.而正常儿童中无一例有较高的T-γ。值在血液中(P=0.02，费雪S精确检验)。这可能表明可能存在一个单独的JRA患者组，具有高T-γ。滑膜室中的浓度。在6名JRA患者中，进一步分析了T.Gamma。与相应的外周血(P<；0.05，S签名检验)和正常儿童外周血(P<；0.05，Mann-Whitney U检验)相比，滑膜室中的V.delta.1+细胞明显占优势。在这6例患者中，滑膜间极早期活化抗原CD69的Tγβ细胞表达显著高于外周血(P<0.05，P<0.05，S签名试验)。滑膜T.Gamma..三角洲。也可以检测到表达人类白细胞抗原-DR和白介素2受体的细胞，而不是外周血中没有T-γ。可以找到表达这些抗原的细胞。这些数据表明滑膜T.Gamma..增量。细胞在体内已经被激活。
Using the anti-TcR.gamma./.delta.-1 monoclonal antibody and flow cytometry, we examined the number of Y.gamma..delta. cells in paired samples of peripheral blood and synovial fluid or tissue from 24 children with juvenile rheumatoid arthritis (JRA), five adult patients with JRA, and 14 patients with rheumatoid arthritis (RA). No significant difference was found in the synovial compartment T.gamma..delta. values compared with the blood in JRA, adult JRA, or RA patients. Nor was any significant difference found in the peripheral blood or synovial compartment T.gamma..delta. values in any of the three patient groups compared with the peripheral blood of normal controls. However, seven of the children with JRA had very high T.gamma..delta. values in the synovial compartment while none of the normal children had high T.gamma..delta. values in the blood (P = 0.02, Fisher''s exact test). This may indicate a possible separate JRA patient group with high T.gamma..delta. levels in the synovial compartment. In six JRA patients further analysed for T.gamma..delta. subpopulations, a significant predominance of V.delta.1+ cells was found in the synovial compartment compared with the corresponding peripheral blood samples (P < 0.05, Wilcoxon''s signed test) and with peripheral blood of child controls (P < 0.05, Mann-Whitney U test). In these six patients, the T.gamma..delta.-cell expression of the very early activation antigen CD69 were significantly higher (P < 0.05, Wilcoxon''s signed test) in the synovial compartment compared with the peripheral blood. Synovial T.gamma..delta. cells expressing HLA-DR and interleukin 2 receptors could also be detected, in contrast to the peripheral blood in which no T.gamma..delta. cells expressing these antigens could be found. These data suggest that the synovial T.gamma..delta. cells had been activated in vivo.