Mammalian Target of Rapamycin Signaling Pathway Changes with Intestinal Epithelial Cells Renewal Along Crypt-Villus Axis
Mammalian Target of Rapamycin Signaling Pathway Changes with Intestinal Epithelial Cells Renewal Along Crypt-Villus Axis
复制标题
哺乳动物雷帕霉素靶标信号通路随肠上皮细胞沿隐窝绒毛轴更新的变化
DOI：
10.1159/000445665
复制
发表时间：
2016-01-01
影响因子：
--
通讯作者：
Yin, Yulong
中科院分区：
文献类型：
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作者：
Yang, Huansheng;Xiong, Xia;Yin, Yulong
背景/目标：了解肠上皮细胞更新的调控机制是研究肠粘膜发育和功能调控及相关疾病的基础。雷帕霉素靶蛋白（mTOR）信号通路是一条整合细胞内外信号的信号通路，参与调控细胞内的各种重要过程。本实验旨在检测mTOR信号通路与肠上皮细胞沿着隐窝-绒毛轴（CVA）更新之间的相关性。研究方法：从沿着CVA的仔猪空肠中依次分离肠上皮细胞，并测量分离的细胞中参与细胞周期、mTOR信号通路、基因表达和抗氧化能力的蛋白质的量或磷酸化水平。结果：从腺窝到绒毛尖，参与细胞周期的蛋白质数量逐渐减少。肠上皮细胞中mTOR信号通路相关蛋白的数量或磷酸化水平主要在沿着CVA成熟过程中下降。与基因表达有关的蛋白质含量和抗氧化能力也从腺窝到绒毛顶部逐渐降低。结论：这些结果表明，mTOR信号通路可能沿着CVA参与调节肠上皮细胞的更新，部分可能是通过影响肠上皮细胞的抗氧化能力和基因表达来实现的。需要进一步的组织学验证来确认本实验的结果。
Background/Aims: Understanding the mechanism that involves in regulating epithelial cells renewal is the fundamental of regulating intestinal mucosa development and functions and related diseases. The mechanistic target of rapamycin (mTOR) signaling pathway involves in controlling various major processes by integrating intracellular and extracellular cues. The present experiment was conducted to test the correlation between the mTOR signaling pathway and intestinal epithelial cells renewal along crypt-villus axis (CVA). Methods: Intestinal epithelial cells were sequentially isolated from the jejunum of piglets along CVA, and the amount or phosphorylation level of proteins involved in cell cycle, mTOR signaling pathway, gene expression, and the antioxidant capacity in the isolated cells were measured. Results: The results showed that the amount of proteins involved in cell cycle decreased from crypt to villus tip. The amount or phosphorylation level of proteins related to mTOR signaling pathway in intestinal epithelial cells mainly decreased during maturation along CVA. The amount of proteins involved in gene expression and the antioxidant capacity also decreased from crypt to the top of villi. Conclusions: These results indicate that the mTOR signaling pathway may be involved in regulating the intestinal epithelial cells renewal along CVA and it may partly through affecting the antioxidant capacity and gene expression of intestinal epithelial cells. Further histological verification is needed to confirm the results of the present experiments.