Noninfectious virus-like particles produced by Moloney murine leukemia virus-based retrovirus packaging cells deficient in viral envelope become infectious in the presence of lipofection reagents.
Noninfectious virus-like particles produced by Moloney murine leukemia virus-based retrovirus packaging cells deficient in viral envelope become infectious in the presence of lipofection reagents.
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由缺乏病毒包膜的莫洛尼鼠白血病病毒为基础的逆转录病毒包装细胞产生的非感染性病毒样颗粒在脂转染试剂存在下变得具有感染性。
DOI：
10.1073/pnas.94.20.10803
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发表时间：
1997
影响因子：
11.1
通讯作者：
Friedmann,T
中科院分区：
文献类型：
--
作者：
Sharma,S;Murai,F;Miyanohara,A;Friedmann,T
Retrovirus packaging cell lines expressing the Moloney murine leukemia virusgagandpolgenes but lacking virus envelope genes produce virus-like particles constitutively, whether or not they express a transcript from an integrated retroviral provirus. In the absence of a proviral transcript, the assembled particles contain processedgagand reverse transcriptase, and particles made by cells expressing an integratedlacZprovirus also contain viral RNA. The virus-like particles from both cell types are enveloped and are secreted/budded into the extracellular space but are noninfectious. Their physicochemical properties are similar to those of mature retroviral particles. The noninfectiousgag pol RNAparticles can readily be made infectious by the addition of lipofection reagents to produce preparations with titers of up to 105colony-forming units per ml.
