Low Expression of MicroRNA-126 is Associated with Poor Prognosis in Colorectal Cancer
Low Expression of MicroRNA-126 is Associated with Poor Prognosis in Colorectal Cancer
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MicroRNA-126 的低表达与结直肠癌的不良预后相关
DOI：
10.1002/gcc.22146
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发表时间：
2014-04-01
影响因子：
3.7
通讯作者：
Luo, Hesheng
中科院分区：
文献类型：
--
作者：
Liu, Yaling;Zhou, Yu;Luo, Hesheng
据报道，MicroRNA‐126 （miR‐126）是一种在结直肠癌（CRC）细胞中靶向scxcr4的肿瘤抑制因子。这项研究调查了miR - 126是否对结直肠癌患者的预后有影响。采用实时定量PCR检测92对结直肠癌及邻近非肿瘤组织中MiR - 126和cxcr4mrna的表达，采用免疫组化（IHC）和Western blotting检测CXCR4蛋白的表达。确定miR - 126和CXCR4蛋白表达与临床病理特征和总生存率的相关性。MiR - 126在高水平表达cxcr4mrna的结直肠癌组织中下调。免疫组化和Western blotting检测到CXCR4蛋白在结直肠癌组织中高表达。miR - 126和CXCR4蛋白在结直肠癌组织中的表达呈负相关。此外，低miR - 126和高CXCR4蛋白表达与远处转移、临床TNM分期和较差的生存率相关。多因素分析表明miR - 126是总生存的独立预后因素，提示其作为结直肠癌患者预后预测因子的临床意义。©2014 Wiley期刊公司
MicroRNA‐126 (miR‐126) has been reported to be a tumor suppressor that targetsCXCR4in colorectal cancer (CRC) cells. This study investigated whether miR‐126 has any prognostic impact in patients with CRC. MiR‐126 andCXCR4mRNA expression in 92 pairs of CRC and adjacent nontumorous tissues was examined using quantitative real‐time PCR, and CXCR4 protein expression was assessed by immunohistochemistry (IHC) and Western blotting. The correlation between miR‐126 and CXCR4 protein expression and clinicopathological features and overall survival rate was determined. MiR‐126 was downregulated in CRC tissues that expressed high levels ofCXCR4mRNA. IHC and Western blotting detected high expression of CXCR4 protein in CRC tissues. An inverse correlation was observed between miR‐126 and CXCR4 protein expression in CRC tissues. Moreover, low miR‐126 and high CXCR4 protein expression was associated with distant metastasis, clinical TNM stage, and poor survival. Multivariate analysis indicated that miR‐126 was an independent prognostic factor for overall survival, suggesting its clinical significance as a prognostic predictor in CRC patients. © 2014 Wiley Periodicals, Inc.