Human immunodeficiency virus mutations during the first month of infection are preferentially found in known cytotoxic T-lymphocyte epitopes
Human immunodeficiency virus mutations during the first month of infection are preferentially found in known cytotoxic T-lymphocyte epitopes
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DOI：
10.1128/jvi.79.17.11523-11528.2005
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发表时间：
2005-09-01
影响因子：
5.4
通讯作者：
Delwart, E
中科院分区：
文献类型：
--
作者：
Bernardin, F;Kong, D;Delwart, E
人类免疫缺陷病毒（HIV）基因组的全蛋白编码区进行测序使用血浆收集从9名非洲裔美国人血清转换前和7至28天后。在这些受试者中，有7人以每年2%的全基因组速率出现HIV突变。将这些受试者中非同义（NS）HIV突变的位置与Los Alamos国家实验室HIV免疫学数据库中报告的潜在HLA-A和B型限制性CTL表位进行比较。在先前报道的CTL表位中发现了统计学显著（P < 0.005）的早期NS突变（13.5%）。因此，病毒测序和报告的CTL表位数据库分析支持一个模型，其中一个显着比例的非常早期的非同义HIV突变的CTL选择。
The full protein coding region of human immunodeficiency virus (HIV) genomes were sequenced using plasma collected from nine African-Americans prior to seroconversion and 7 to 28 days later. HIV mutations emerged in seven of these subjects at a genomewide rate of 2% per year. The location of nonsynonymous (NS) HIV mutations within these subjects was compared to their potential HLA-A and B types restricted CTL epitopes reported in the Los Alamos National Laboratory HIV immunology database. A statistically significant (P < 0.005) number of the early NS mutations (13.5%) were found within previously reported CTL epitopes. A virus sequencing and reported CTL epitopes database analysis therefore support a model where a significant proportion of very early nonsynonymous HIV mutations are selected by CTL.