Clenbuterol Changes Phosphorylated FOXO1 Localization and Decreases Protein Degradation in the Skeletal Muscle of Neonatal Chicks.
Clenbuterol Changes Phosphorylated FOXO1 Localization and Decreases Protein Degradation in the Skeletal Muscle of Neonatal Chicks.
复制标题
克伦特罗改变新生雏鸡骨骼肌中的磷酸化 FOXO1 定位并减少蛋白质降解。
DOI：
10.1080/09168451.2016.1158629
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Akira Ohtsuka.
中科院分区：
文献类型：
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作者：
Saki Shimamoto;Daichi Ijiri;Kazuki Nakashima;Mana Kawaguchi;Yoshitaka Ishimaru;Airi Furukawa;Akira Ohtsuka.
为了研究细胞内信号转导机制，克伦特罗减少肌肉蛋白质降解，我们研究了磷酸化水平和新生鸡缝匠肌FOXO 1的细胞内定位。对1日龄雏鸡单次腹腔注射克伦特罗（0.1 mg/kg体重）。注射克伦特罗后3 h，缝匠肌AKT蛋白磷酸化。巧合的是，克伦特罗增加磷酸化FOXO 1蛋白的胞浆水平，而减少缝匠肌中FOXO 1蛋白的核水平。此外，克仑特罗减少肌肉特异性泛素连接酶（atrogin-1/MAFbx和MuRF 1）在缝匠肌伴随着血浆3-甲基组氨酸浓度降低，肌肉蛋白质降解的指数，在注射后3小时的mRNA的表达。这些结果表明，在缝匠肌，克伦特罗改变了磷酸化FOXO 1的细胞内定位，从而通过抑制编码肌肉特异性泛素连接酶的基因的表达减少蛋白质降解。
To investigate the intracellular signaling mechanisms by which clenbuterol reduces muscle protein degradation, we examined the phosphorylation level and intracellular localization of FOXO1 in the sartorius muscle of neonatal chicks. One-day-old chicks were given a single intraperitoneal injection of clenbuterol (0.1 mg/kg body weight). Three hours after injection, AKT protein was phosphorylated in the sartorius muscle by clenbuterol injection. Coincidentally, clenbuterol increased cytosolic level of phosphorylated FOXO1 protein, while it decreased nuclear level of FOXO1 protein in the sartorius muscle. Furthermore, clenbuterol decreased the expression of mRNAs for muscle-specific ubiquitin ligases (atrogin-1/MAFbx and MuRF1) in the sartorius muscle accompanied by decreased plasma 3-methylhistidine concentration, an index of muscle protein degradation, at 3 h after injection. These results suggested that, in the sartorius muscle of the chicks, clenbuterol changed the intracellular localization of phosphorylated FOXO1, and consequently decreased protein degradation via suppressing the expression of genes encoding muscle-specific ubiquitin ligases.