High-speed free-flow electrophoresis on chip
High-speed free-flow electrophoresis on chip
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DOI：
10.1021/ac0345190
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发表时间：
2003-11-01
影响因子：
7.4
通讯作者：
Manz, A
中科院分区：
文献类型：
--
作者：
Zhang, CX;Manz, A
研制了一种用于自由流电泳（FFE）连续分离的微流控装置。使用2 nL/s的样品流速，在75 ms内将两种荧光试剂的混合物分离成两种组分流。样品在整个分离室中的停留时间为2 s。分离床体积为0.2穆尔。该芯片也被用于自由流动电泳的荧光素-5-异硫氰酸酯标记的氨基酸在水溶液和二元介质。短的停留时间和小的样品流速使得FFE芯片可用于在线监测生产细粉和其他化学或生物化学过程。自制的芯片由1.5 mm厚的普通玻璃基板和0.3 mm厚的PDMS层组成，具有微加工通道。本文提出的通道设计是通用的。使用相同种类的PDMS基板，可以根据在PDMS基板上切割出的储存器的位置来制造用于各种目的的芯片。所提出的结果验证了缩放定律，并允许预测的FFE性能相媲美，现在是最先进的毛细管电泳芯片。
A microfluidic device has been developed for continuous separation in free-flow electrophoresis (FFE) mode. A mixture of two fluorescent reagents is separated into two component streams in 75 ms using a sample flow rate of 2 nL/s. The residence time of sample in the whole separation compartment is 2 s. The separation bed volume is 0.2 muL. The chip has also been used for free-flow electrophoresis of fluorescein-5-isothiocyanate-labeled amino acids in both aqueous and binary media. The short residence time and small sample flow rate make the FFE chip feasible for on-line monitoring on production fines and other chemical or biochemical processes. The in-house-made chip was composed of a plain glass substrate of 1.5-mm thickness and a PDMS layer of 0.3-mm, thickness with micromachined channels. The channel design presented in this paper is versatile. With the same kind of PDMS substrates, chips for various purposes can be made depending on the locations of the reservoirs, which are cut out on the PDMS substrate. The results presented verify the scaling laws and allow prediction of FFE performances comparable to what is now state of the art on capillary electrophoresis chips.