Expression of mRNA for Phospholipase A2, Cyclooxygenases, and Lipoxygenases in Cultured Human Umbilical Vascular Endothelial and Smooth Muscle Cells and in Biopsies from Umbilical Arteries and Veins
Expression of mRNA for Phospholipase A2, Cyclooxygenases, and Lipoxygenases in Cultured Human Umbilical Vascular Endothelial and Smooth Muscle Cells and in Biopsies from Umbilical Arteries and Veins
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培养的人脐血管内皮细胞和平滑肌细胞以及脐动脉和静脉活检中磷脂酶 A2、环氧合酶和脂氧合酶 mRNA 的表达
DOI：
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发表时间：
1998
影响因子：
1.7
通讯作者：
A. Sirsjö
中科院分区：
文献类型：
--
作者：
Martin Öst;E. Uhl;M. Carlsson;A. Gidlöf;P. Söderkvist;A. Sirsjö
花生四烯酸（AA）由磷脂酶A2（PLA 2）释放，然后由环氧合酶（考克斯）和脂氧合酶（LOX）转化为具有血管活性和炎症性的类花生酸。这些类二十烷酸是血管通透性、血流、局部促凝血活性和抗凝活性的重要旁分泌调节剂，并且它们在局部炎症反应中起主要作用。我们已经调查了存在的mRNA的PLA 2和异构体的考克斯和LOX在人内皮细胞（EC）和人平滑肌细胞（SMC）在培养和血管活检的人脐静脉（HUVB）和动脉（HUAB）通过使用逆转录-聚合酶链反应（RT-PCR）技术。结果显示在培养的EC和SMC中以及来自HUAB和HUVB的血管壁活检中可检测水平的PLA 2 IV型（cPLA 2）。PCR分析显示培养的EC和SMC的考克斯-1和考克斯-2的水平高于HUAB和HUVB的血管壁活检。这表明EC和SMC培养物中考克斯-1和考克斯-2的天然表达与来自完整血管壁的活检组织中的表达相比存在差异。培养的EC和SMC不表达5-LOX的mRNA，然而，在血管壁活检中表达。这说明5-LOX在脐静脉和动脉的血管壁中的SMC中的组成型表达，即体内表达。因此，体外研究EC和血管SMC中组成型考克斯和LOX表达的结果不能简单地外推以代表体内条件。
Arachidonic acid (AA) is released by phospholipase A2 (PLA2) and then converted into vasoactive and inflammatory eicosanoids by cyclooxygenases (COX) and lipoxygenases (LOX). These eicosanoids are important paracrine regulators of vascular permeability, blood flow, local pro- and anticoagulant activity and they play a major role in the local inflammatory response. We have investigated the presence of mRNAs for PLA2 and for isoforms of COX and LOX in both human endothelial cells (EC) and in human smooth muscle cells (SMC) in culture and in vascular biopsies of human umbilical veins (HUVB) and arteries (HUAB) by using the reversed transcription-polymerase chain reaction (RT-PCR) technique. Results show detectable levels of PLA2 type IV (cPLA2) in cultured EC and SMC and in vascular wall biopsies from HUAB and HUVB. The cultured EC and SMC demonstrate higher levels of both COX-1 and COX-2 with PCR analyses than do vascular wall biopsies from HUAB and HUVB. This indicates a difference in the native expression of COX-1 and COX-2 in cultures of EC and SMC compared to that in biopsies from intact vessel walls. The EC and SMC in culture do not express mRNA for 5-LOX, that was, however, expressed in the vascular wall biopsies. This speaks in favour of a constitutive, i.e. in vivo expression of 5-LOX in SMC in the vascular wall of both umbilical vein and arteries. Thus results from in vitro studies of constitutive COX and LOX expression in EC and vascular SMC in culture cannot simply be extrapolated to represent in vivo conditions.
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DOI：
10.1016/s0021-9258(18)54392-1
发表时间：
1991-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Hla;T. Maciag
通讯作者：
T. Hla;T. Maciag
通过竞争性聚合酶链反应定量分析血管前列腺素 H 合酶 mRNA 水平。
DOI：
--
发表时间：
1993
期刊：
The Journal of laboratory and clinical medicine
影响因子：
--
作者：
Zyglewska,T;Wu,LC;Xu,XM;Conlan,MG;Wang,LH;Wu,KK
通讯作者：
Wu,KK
来自牛内皮细胞的激素调节和颗粒相关磷脂酶 A2 的表征。
DOI：
--
发表时间：
1993
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Paglin,S;Roy,R;Polgar,P
通讯作者：
Polgar,P
DOI：
10.1016/s0006-291x(88)80271-7
发表时间：
1988-12
影响因子：
3.1
作者：
Elliott Sigal;Charles S. Craik;Ella Highland;D. Grunberger;Lawrence L. Costello;R. A. Dixon;J. A. Nadel
通讯作者：
Elliott Sigal;Charles S. Craik;Ella Highland;D. Grunberger;Lawrence L. Costello;R. A. Dixon;J. A. Nadel
IL-1 α 诱导环氧合酶-2。
DOI：
--
发表时间：
1994
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ristimäki,A;Garfinkel,S;Wessendorf,J;Maciag,T;Hla,T
通讯作者：
Hla,T