Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain
Comprehensive proteomic profiling of the membrane constituents of a Mycobacterium tuberculosis strain
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DOI：
10.1074/mcp.m300060-mcp200
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发表时间：
2003-12-01
影响因子：
7
通讯作者：
Chen, X
中科院分区：
文献类型：
--
作者：
Gu, S;Chen, J;Chen, X
结核分枝杆菌是一种引起人类结核病的传染性微生物。众所周知，病原体的细胞膜富含可能的诊断和治疗蛋白质靶点。为了补充结核分枝杆菌基因组，我们利用一维SDS凝胶与微毛细管液相色谱-纳米喷雾-串联质谱仪耦合的分析平台对结核分枝杆菌H37Rv菌株的膜蛋白部分进行了分析。结果，739种蛋白质已被两个或更多不同的肽序列鉴定，并已被表征。有趣的是，近450种蛋白质代表了新的鉴定，其中79种是膜蛋白，其中100多种是膜相关蛋白。对鉴定的蛋白进行了详细的理化性质研究，并根据Sanger Institute基因功能分类对其进行了分类，得到了其生物学功能。发现许多膜蛋白参与细胞包膜，并在本研究中发现了具有能量代谢功能的蛋白。
Mycobacterium tuberculosis is an infectious microorganism that causes human tuberculosis. The cell membranes of pathogens are known to be rich in possible diagnostic and therapeutic protein targets. To compliment the M. tuberculosis genome, we have profiled the membrane protein fraction of the M. tuberculosis H37Rv strain using an analytical platform that couples one-dimensional SDS gels to a microcapillary liquid chromatography-nanospray-tandem mass spectrometer. As a result, 739 proteins have been identified by two or more distinct peptide sequences and have been characterized. Interestingly, similar to450 proteins represent novel identifications, 79 of which are membrane proteins and more than 100 of which are membrane-associated proteins. The physicochemical properties of the identified proteins were studied in detail, and then biological functions were obtained by sorting them according to Sanger Institute gene function category. Many membrane proteins were found to be involved in the cell envelope, and those proteins with energy metabolic functions were also identified in this study.