Analysis of TGFβ3 gene expression and protein levels in human bone and serum
Analysis of TGFβ3 gene expression and protein levels in human bone and serum
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DOI：
10.1055/s-2001-14830
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发表时间：
2001-01-01
影响因子：
1.8
通讯作者：
Pfeiffer, AFH
中科院分区：
文献类型：
--
作者：
Hering, S;Isken, F;Pfeiffer, AFH
最近的数据表明，TGF β 3，TGF β-同种型的一个成员，在骨重建中具有重要作用。我们建立了一种高特异性的ELISA方法用于定量检测骨和血液中TGF β 3的含量，并建立了RT-PCR结合HPLC检测89例人骨组织中TGF β 3 mRNA的方法。TGF β 3蛋白水平范围为30 - 66 pg/mg骨（平均36.6 +/-1.03 pg/mg），血清中为30 - 1910 pg/ml（平均128.9 +/- 35.9 pg/ml）。血清和骨组织中TGFB 3 mRNA表达和蛋白水平随年龄增长而下降。在不同的位点没有检测到TGF β 3 mRNA表达或蛋白质含量的特定负载或位点特异性分布，表明没有机械调节。血清中TGF β 3蛋白水平与骨中TGF β 3 mRNA表达相关（p= 0.0027; r=0.49）。相反，储存在骨基质中的TGF β 3蛋白水平与TGF β 3 mRNA无关，反映了骨重建期间TGF β 3沉积的长期过程。值得注意的是，TGF β 3血清水平与血清中的IGF-Id和骨钙素水平高度相关。我们的结论是，TGF β 3在人循环中的显着量，这似乎是代表TGF β 3在骨组织中的表达，并可能部分来自骨。TGFB 3与IGF-I的高度相关性表明了平行的系统性调节原则。
Recent data indicate that TGF beta3, one member of the TGF beta -isoforms, has an important role in bone remodeling. Up to date little is known about the expression and regulation of TGF beta3 in man. We established a highly specific ELISA for quantitative measurement of TGF beta3 in bone and blood samples and a RT-PCR in combination with HPLC for detection and quantification of TGF beta3 mRNA in 89 human bone samples. Levels of TGF beta3 protein ranged between 30 and 66 pg/mg bone (mean 36,6 +/- 1,03 pg/mg) and between 30 and 1910 pg/ml in serum (mean 128,9 +/- 35.9 pg/ml). TGFB3 mRNA expression as well as protein levels in serum and in bone declined age dependently. No specific load- or site-specific distribution of TGF beta3 mRNA expression or protein content was detected at different sites indicating an absence of mechanical regulation. Protein levels of TGF beta3 in serum correlated with TGFB3 mRNA expression in bone (p= 0.0027; r=0.49). By contrast, TGF beta3 protein levels stored in the bone matrix were not related to TGF beta3 mRNA reflecting the long term process of TGF beta3 deposition during bone remodeling. Notably TGF beta3 serum levels were highly correlated with IGF-Id and osteocalcin levels in serum. We conclude that TGF beta3 in man circulates in significant amounts which appears to be representative for TGF beta3 expression in bone tissue and may be in part derived from bone. The high correlation of TGFB3 with IGF-I suggests parallel systemic principles of regulation.