Anaerobic bacteria cultured from cystic fibrosis airways correlate to milder disease: a multisite study
Anaerobic bacteria cultured from cystic fibrosis airways correlate to milder disease: a multisite study
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DOI：
10.1183/13993003.00242-2018
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发表时间：
2018-07-01
影响因子：
24.3
通讯作者：
Tunney, Michael M.
中科院分区：
文献类型：
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作者：
Muhlebach, Marianne S.;Hatch, Joseph E.;Tunney, Michael M.
使用扩展培养方法对三个囊性纤维化（CF）中心的呼吸道样本中的厌氧菌和需氧菌进行定量。年龄1-69岁且临床稳定的受试者提供痰液（n=200）或支气管肺泡灌洗（n=55）。分别从59%和95%的样本中培养出18个厌氧属和39个需氧属; 57个属中有16个在各中心的患病率>= 5%。在痰样本中使用共现网络进行的微生物群落分析显示，包括厌氧在内的口腔细菌分组，而典型的CF病原体形成不同的实体。假单胞菌与较差的营养和F508 del基因型相关，而厌氧菌的患病率与胰腺功能充足、较好的营养和较好的肺功能正相关。较高的总厌氧菌/总需氧菌CFU比与胰腺功能充足和营养较好相关。与以假单胞菌为主且F508 del纯合受试者比例相似的组相比，根据因素分析分组的受试者中，厌氧菌相对于需氧菌占主导地位，其疾病较轻。总而言之，厌氧细菌出现在早期。在产瘤受试者中，厌氧菌与较轻的疾病相关，这表明在产瘤CF受试者中可能不需要靶向根除厌氧菌。
Anaerobic and aerobic bacteria were quantitated in respiratory samples across three cystic fibrosis (CF) centres using extended culture methods. Subjects aged 1-69 years who were clinically stable provided sputum (n=200) or bronchoalveolar lavage (n=55). 18 anaerobic and 39 aerobic genera were cultured from 59% and 95% of samples, respectively; 16 out of 57 genera had a >= 5% prevalence across centres.Analyses of microbial communities using co-occurrence networks in sputum samples showed groupings of oral, including anaerobic, bacteria, whereas typical CF pathogens formed distinct entities. Pseudomonas was associated with worse nutrition and F508del genotype, whereas anaerobe prevalence was positively associated with pancreatic sufficiency, better nutrition and better lung function. A higher total anaerobe/total aerobe CFU ratio was associated with pancreatic sufficiency and better nutrition. Subjects grouped by factor analysis who had relative dominance of anaerobes over aerobes had milder disease compared with a Pseudomonas-dominated group with similar proportions of subjects that were homozygous for F508del.In summary, anaerobic bacteria occurred at an early age. In sputum-producing subjects anaerobic bacteria were associated with milder disease, suggesting that targeted eradication of anaerobes may not be warranted in sputum-producing CF subjects.