Aerobic Degradation of Sulfadiazine by Arthrobacter spp.: Kinetics, Pathways, and Genomic Characterization
Aerobic Degradation of Sulfadiazine by Arthrobacter spp.: Kinetics, Pathways, and Genomic Characterization
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DOI：
10.1021/acs.est.6b02231
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发表时间：
2016-09-06
影响因子：
11.4
通讯作者：
Zhang, Tong
中科院分区：
文献类型：
--
作者：
Deng, Yu;Mao, Yanping;Zhang, Tong
从磺胺嘧啶（SDZ）富集活性污泥中分离到2株节杆菌属的好氧磺胺嘧啶降解菌D2和D4。两株菌对SDZ的降解均符合一级降解动力学。菌株D2和菌株D4完全降解SDZ的半衰期分别为11.3 h和46.4 h。当葡萄糖作为共底物引入时，降解动力学由非生长型转变为生长型，适应期后降解速率加快。这两株菌均能通过3条平行途径降解SDZ，产生12种降解产物，其中2-氨基-4-羟基嘧啶是嘧啶环断裂的主要中间产物。与以往报道的5株节杆菌相比，D2和D4是唯一能降解SDZ的节杆菌。D2和D4的基因组草案，具有相同的99.7%的完整性，与其他相关物种的基因组进行了比较。总的来说，这两个分离株与s-三嗪降解节杆菌属AK-YNIO和磺胺降解细菌微杆菌属C448具有高度的基因组相似性。此外，两个基因组包含一些显著的差异区域，可能携带参与磺胺降解的功能基因。
Two aerobic sulfadiazine (SDZ) degrading bacterial strains, D2 and D4, affiliated with the genus Arthrobacter, were isolated from SDZ-enriched activated sludge. The degradation of SDZ by the two isolates followed first-order decay kinetics. The half-life time of complete SDZ degradation was 11.3 h for strain D2 and 46.4 h for strain D4. Degradation kinetic changed from nongrowth to growth-linked when glucose was introduced as the cosubstrate, and accelerated biodegradation rate was observed after the adaption period. Both isolates could degrade SDZ into 12 biodegradation products via 3 parallel pathways, of which 2-amino-4-hydroxypyrimidine was detected as the principal intermediate product toward the pyrimidine ring cleavage. Compared with five Arthrobacter strains reported previously, D2 and D4 were the only Arthrobacter strains which could degrade SDZ as the sole carbon source. The draft genomes of D2 and D4, with the same completeness of 99.7%, were compared to other genomes of related species. Overall, these two isolates shared high genomic similarities with the s-triazine-degrading Arthrobacter sp. AK-YNIO and the sulfonamide-degrading bacteria Microbacterium sp. C448. In addition, the two genomes contained a few significant regions of difference which may carry the functional genes involved in sulfonamide degradation.