Sources of variability in data from a lacl transgenic mouse mutation assay
Sources of variability in data from a lacl transgenic mouse mutation assay
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lacl 转基因小鼠突变测定数据的变异性来源
DOI：
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发表时间：
1994
影响因子：
2.8
通讯作者：
M. Shelby
中科院分区：
文献类型：
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作者：
W. Piegorsch;A. Lockhart;B. Margolin;K. Tindall;N. Gorelick;J. Short;G. Carr;E. Thompson;M. Shelby
本文详细讨论了以大肠杆菌为靶基因的转基因小鼠突变试验的实验特点。实验方案中可变性的来源可能会影响观察结果的统计性质，目的是确定观察到的突变部分中过度变化的来源。来源包括板对板（包装内）、包对包（动物内）和动物对动物（研究内）的可变性。使用不同的统计方法对来自两个实验室的数据进行评估，以确定过度变异性。结果表明，除了从组织中分离出试验动物的基因组DNA后较长时间（例如50 - 90天）储存的情况外，只有零散的过度变异模式。我们鼓励进一步的研究来检验这种与时间/储存相关的效应的有效性和含义。
Experimental features of a transgenic mouse mutation assay based on a lacl target transgene from Escherichia coli are considered in detail. Sources of variability in the experimental protocol that can affect the statistical nature of the observations are examined with the goal of identifying sources of excess variation in the observed mutant fractions. The sources include plate‐to‐plate (within packages), package‐to‐package (within animals), and animal‐to‐animal (within study) variability. Data from two laboratories are evaluated, using various statistical methods to identify excess variability. Results suggest only scattered patterns of excess variability, except possibly in those cases where genomic DNA from test animals is stored for extended periods (e.g., > 90 days) after isolation from tissues. Further study is encouraged to examine the validity and implications of this time/storage‐related effect.
短期体内诱变测定的开发：甲基化对从转基因小鼠中恢复 lambda 噬菌体穿梭载体的影响。
影响因子：
14.9
作者：
Kohler,SW;Provost,GS;Kretz,PL;Dycaico,MJ;Sorge,JA;Short,JM
通讯作者：
Short,JM
影响因子：
2.9
作者：
FREDERICO, LA;KUNKEL, TA;SHAW, BR
通讯作者：
SHAW, BR