Functional recovery and expression of GDNF seen in photochemically induced cerebral infarction
Functional recovery and expression of GDNF seen in photochemically induced cerebral infarction
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DOI：
10.1080/00207450600582124
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发表时间：
2007-03-01
影响因子：
2.2
通讯作者：
Kawahira, Kazumi
中科院分区：
文献类型：
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作者：
Horinouchi, Keisuke;Ikeda, Satoshi;Kawahira, Kazumi
胶质细胞源性神经营养因子（Glial cell line-derived neurotrophic factor，GDNF）是一种重要的神经营养因子，参与神经元的存活和增殖。然而，很少有研究GDNF与脑梗死后功能恢复的关系的报道。作者研究了光化学诱导的脑梗死大鼠在功能恢复过程中GDNF蛋白表达的变化。梗死后前14天的功能恢复使用横梁行走试验进行评估。于梗死后24 h、72 h、7 d、14 d分别计数梗死灶周围GDNF样免疫反应阳性细胞数。脑切片的免疫组织化学分析表明，GDNF样免疫反应细胞的表达显着增加，颞叶皮质，直到7天的同侧梗死，并减少了14天。同样，瘫痪的功能恢复是实质性的，直到梗死后7天，之后的改善是轻微的。因此，GDNF蛋白的表达可能与瘫痪功能的恢复有一定的关系。GDNF有望成为脑梗死的治疗药物。
Glial cell line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor involved in the survival and proliferation of neurons. However, there have been few reports examining the relationship between GDNF and functional recovery after cerebral infarction. The authors investigated the change in the expression of GDNF proteins during functional recovery in rats following photochemically induced cerebral infarctions. Functional recovery for the first 14 days after the infarction was evaluated using a beam-walking test. The number of GDNF-like immunoreactive cells around the infarction were counted at various times (24 h, 72 h, 7 days, and 14 days) post-infarction. Immunohistochemical analysis of brain sections showed that the expression of GDNF-like immunoreactive cells was significantly increased in the temporal cortex until 7 days on the side ipsilateral to the infarction, and had decreased by 14 days. Likewise, the functional recovery of paralysis was substantial until 7 days post-infarction, after which the improvement was mild. Therefore, the expression of GDNF protein might have some relationship with the functional recovery of paralysis. There are great hopes that GDNF could be used as a therapeutic agent for cerebral infarction.