Temperature Depended Role of Shigella flexneri Invasion Plasmid on the Interaction with Acanthamoeba castellanii.
Temperature Depended Role of Shigella flexneri Invasion Plasmid on the Interaction with Acanthamoeba castellanii.
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DOI：
10.1155/2012/917031
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发表时间：
2012
影响因子：
3.4
通讯作者：
Abd H
中科院分区：
文献类型：
--
作者：
Saeed A;Johansson D;Sandström G;Abd H
福氏志贺菌是一种革兰氏阴性菌，可引起人类肠道疾病志贺菌病。入侵所需的毒力基因聚集在一个大的220 kb质粒上，该质粒编码三型分泌系统（TTSS）装置和毒力因子，如粘附和入侵质粒抗原（Ipa）。这种细菌通过受污染的食物、水或人与人之间传播。阿米巴原虫是自由生活的阿米巴原虫（弗拉），存在于不同的环境中，并与各种水源隔离。不同的细菌与弗拉的相互作用不同，如土拉热弗朗西丝氏菌、霍乱弧菌、宋内志贺菌和志贺菌。紫茎泽兰能在A. castellanii。与此相反，铜绿假单胞菌诱导坏死和凋亡来杀死A。castellanii。本研究的目的是探讨S. flexneri对A. Castellanii在两个不同的温度下。A. castellanii在不存在或存在野生型、IpaB突变体或质粒固化菌株S.在30°C和37°C下培养福氏杆菌，并通过细菌和阿米巴的活菌计数、电子显微镜、流式细胞术和统计分析来分析相互作用。自A. castellanii在30°C时受到抑制，而A.在37°C下通过侵入质粒介导的坏死杀死castellanii。
Shigella flexneri is a Gram-negative bacterium causing the diarrhoeal disease shigellosis in humans. The virulence genes required for invasion are clustered on a large 220 kb plasmid encoding type three secretion system (TTSS) apparatus and virulence factors such as adhesions and invasion plasmid antigens (Ipa). The bacterium is transmitted by contaminated food, water, or from person to person. Acanthamoebae are free-living amoebae (FLA) which are found in diverse environments and isolated from various water sources. Different bacteria interact differently with FLA since Francisella tularensis, Vibrio cholerae, Shigella sonnei, and S. dysenteriae are able to grow inside A. castellanii. In contrast, Pseudomonas aeruginosa induces both necrosis and apoptosis to kill A. castellanii. The aim of this study is to examine the role of invasion plasmid of S. flexneri on the interaction with A. castellanii at two different temperatures. A. castellanii in the absence or presence of wild type, IpaB mutant, or plasmid-cured strain S. flexneri was cultured at 30°C and 37°C and the interaction was analysed by viable count of both bacteria and amoebae, electron microscopy, flow cytometry, and statistical analysis. The outcome of the interaction was depended on the temperature since the growth of A. castellanii was inhibited at 30°C, and A. castellanii was killed by invasion plasmid mediated necrosis at 37°C.