CELLULAR-LOCALIZATION OF VASOPRESSIN V1A RECEPTOR MESSENGER-RIBONUCLEIC-ACID IN ADULT MALE-RAT BRAIN, PINEAL, AND BRAIN VASCULATURE
CELLULAR-LOCALIZATION OF VASOPRESSIN V1A RECEPTOR MESSENGER-RIBONUCLEIC-ACID IN ADULT MALE-RAT BRAIN, PINEAL, AND BRAIN VASCULATURE
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DOI：
10.1210/en.135.4.1511
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发表时间：
1994-10-01
期刊：
影响因子：
4.8
通讯作者：
YOUNG, WS
中科院分区：
文献类型：
--
作者：
OSTROWSKI, NL;LOLAIT, SJ;YOUNG, WS
血管加压素V1 a受体（V1 aR）的成绩单定位于大脑，松果体，和脑血管组织的浅层成年雄性大鼠使用杂交组织化学和[S-35]核糖探针互补的信使核糖核酸（mRNA）编码的第五至midseventh跨膜区的受体。V1 aR mRNA广泛分布于脑内，主要表达于嗅球、海马齿状回和小脑的颗粒细胞层的表层细胞，2）脑内许多解剖学上不同的核团，3）分散于中枢神经系统的单个细胞，4）分布于海马齿状回和小脑的颗粒细胞层的表层细胞。4）脉络丛细胞，嗅球和脚间核内偶见血管，脑实质外血管;（5）部分白色物质结构。在前脑结构中发现了许多表达V1 aR转录本的细胞，包括初级嗅（梨状）皮质，前嗅核和后嗅核;背侧，中间和腹侧外侧隔核;隔伞核和延髓核;以及许多下丘脑区域，其中弓状核，柱头核，视交叉上核，室周核和下丘脑外侧区的下丘脑标记最强烈。细胞表达V1 aR转录普遍存在于整个中脑，脑桥，和延髓区域。低强度的信号被发现在小细胞室旁核和前腹侧核的丘脑，室周器官，包括松果体，和穹窿下器官的细胞。V1 aR转录本一般不检测到实质血管，但可以发现在脚间核和内侧嗅球的大血管;转录本通常检测到血管周围的脑细胞。V1 aR mRNA在脉络丛、主嗅球之间的中线血管内皮细胞和所有脑表面的浅表血管组织中大量表达。这些数据证实了血管/肝型V1 aR基因在脑组织中的存在，并记录了广泛的表达。V1 aR mRNA的分布表明，至少有两种类型的加压素反应细胞在大脑中：一种类型的例子是由经典的轴树突/体突触加压素能终端和第二，血管周围/血管类型，将促进体液加压素能信号在大脑中的外侧隔区神经元。
Vasopressin V1a receptor (V1aR) transcripts were localized in brain, pineal, and superficial brain vascular tissues of adult male rats using hybridization histochemistry and an [S-35]riboprobe complementary to the messenger ribonucleic acid (mRNA) encoding the fifth to the midseventh transmembrane regions of the receptor. V1aR mRNA was extensively distributed throughout brain and was expressed in 1) superficial cells of the granule cell layers of the main olfactory bulb, hippocampal dentate gyrus, and cerebellum; 2) numerous anatomically distinct brain nuclei; 3) isolated cells dispersed throughout the central nervous system; 4) cells of the choroid plexus, occasional blood Vessels in the olfactory bulb and interpeduncular nucleus, and extraparenchymal intracranial vasculature; and 5) some white matter structures. Numerous cells expressing V1aR transcripts were found in forebrain structures, including primary olfactory (piriform) cortex, the anterior and posterior olfactory nuclei; dorsal, intermediate, and ventral lateral septal nuclei; the septo-fimbrial nucleus and accumbens nucleus; and numerous hypothalamic regions with the most intense hypothalamic labeling in the arcuate, stigmoid, suprachiasmatic, and periventricular nuclei and the lateral hypothalamic area. Cells expressing V1aR transcripts were ubiquitous throughout the midbrain, pontine, and medullary regions. A lower intensity signal was found in cells of the parvocellular paraventricular and anteroventral nucleus of the thalamus, circumventricular organs including the pineal, and the subfornical organ. V1aR transcripts were not generally detected in parenchymal vasculature, but could be found over large blood vessels in the interpeduncular nucleus and medial olfactory bulb; transcripts were commonly detected in perivascular brain cells. V1aR mRNA was abundantly expressed by choroid plexus, endothelial cells of midline blood vessels between the main olfactory bulbs, and superficial vascular tissue on all brain surfaces. These data confirm the presence of the vascular/hepatic-type V1aR gene in brain tissue and document an extensive expression. The distribution of V1aR mRNA suggests that there are at least two types of vasopressin-responsive cells in brain: one type exemplified by lateral septal area neurons innervated by classical axodendritic/somatic synaptic vasopressinergic terminals and a second, perivascular/vascular type that would facilitate humoral vasopressinergic signaling in the brain.