Influence of systemic inflammatory response syndrome on host resistance against bacterial infections
Influence of systemic inflammatory response syndrome on host resistance against bacterial infections
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DOI：
10.1097/01.ccm.0000139606.34631.61
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发表时间：
2004-09-01
影响因子：
8.8
通讯作者：
Suzuki, F
中科院分区：
文献类型：
--
作者：
Takahashi, H;Tsuda, Y;Suzuki, F
目的：确定全身炎症反应综合征（SIRS）与宿主针对细菌感染的先天免疫之间的关系。设计：对照动物研究。设置。大学研究实验室。受试者：雄性 BALB/c 小鼠，8-10 周龄。干预措施：将严重 SIRS 小鼠与轻度 SIRS 小鼠在感染粪肠球菌或耐甲氧西林金黄色葡萄球菌 (MRSA) 或暴露于盲肠结扎穿刺 (CLP) 引起的感染并发症后的发病率和死亡率进行比较。此外，在这两组中分析了与这些感染的抗菌先天免疫相关的效应细胞的功能。此外，用来自轻度或重度SIRS小鼠的效应细胞重建SCIDbgMN小鼠（耗尽抗菌效应细胞的SCIDbg小鼠）并暴露于各种感染。测量和主要结果。严重 SIRS 小鼠对粪肠球菌、MRSA 和 CLP 诱导的败血症非常敏感。另一方面，与正常小鼠相比，轻度 SIRS 小鼠对这些感染具有抵抗力。所有接种来自严重SIRS小鼠的腹膜巨噬细胞（PMphi）的SCIDbgMN小鼠在感染粪肠球菌或MRSA后均死亡，而所有接种来自轻度SIRS小鼠的PM的SCIDbgMN小鼠在相同感染后均存活。接种来自正常小鼠的PMphi并暴露于粪肠球菌、MRSA或CLP的SCIDbgMN小鼠的存活率分别为50%、50%或50%。轻度 SIRS 小鼠的 PMphi( 表现出经典活化巨噬细胞 (CAMphi) 的典型特性，而来自重度 SIRS 小鼠的 PMphi 表现出替代活化巨噬细胞 (AAMphi) 的典型特性。结论。Mphi 相关的宿主抗菌先天免疫受 SIRS 水平的影响很大。CAMphi 是针对 E lacalis、MRSA 和 CLP 诱导的脓毒症的抗菌先天免疫的效应细胞，在轻度 SIRS 中被诱导。患有严重 SIRS 的小鼠体内会产生没有抗菌能力的 AAMphi。诱导 CAMphi 可以保护严重 SIRS 患者免受感染。
Objective: To determine the relationship between systemic inflammatory response syndrome (SIRS) and host innate immunities against bacterial infections.Design: Controlled animal study.Setting. University research laboratory.Subjects: Male BALB/c mice, 8-10 wks of age.Interventions: Morbidity and mortality rates of severe SIRS mice were compared with those of mild SIRS mice after infection with Enterococcus faecalis or methicillin-resistant Staphylococcus aureus (MRSA) or exposure to infectious complications induced by cecal ligation and puncture (CLP). In addition, a function of effector cells related to antibacterial innate immunities for these infections was analyzed in these two groups. Furthermore, SCIDbgMN mice (SCIDbg mice depleted of antibacterial effector cells) were reconstituted with effector cells from mild or severe SIRS mice and exposed to various infections.Measurements and Main Results. Severe SIRS mice were greatly susceptible to E faecalis, MRSA, and CLP-induced sepsis. On the other hand, as compared with normal mice, mild SIRS mice were resistant to these infections. All of SCIDbgMN mice inoculated with peritoneal macrophages (PMphi) from severe SIRS mice died after infection with E faecalis or MRSA, whereas all SCIDbgMN mice inoculated with PM( from mild SIRS mice survived after the same infection. SCIDbgMN mice that were inoculated with PMphi from normal mice and exposed to E laecalis, MRSA, or CLP survived at rates of 50%, 50%, or 60%, respectively. PMphi( from mild SIRS mice exhibited typical properties for classically activated macrophages (CAMphi), whereas those from severe SIRS mice exhibited typical properties for alternatively activated macrophages (AAMphi).Conclusions. Mphi-associated host antibacterial innate immunities are greatly influenced by SIRS levels. CAMphi, effector cells for the antibacterial innate immunity against E laecalis, MRSA, and CLP-induced sepsis, are induced in mild SIRS mice. AAMphi( with no antibacterial capabilities are generated in mice with severe SIRS. Induction of CAMphi may protect severe SIRS patients against infections.