Psoralen-mediated photodecontamination of platelet concentrates: inactivation of cell-free and cell-associated forms of human immunodeficiency virus and assessment of platelet function in vivo.
Psoralen-mediated photodecontamination of platelet concentrates: inactivation of cell-free and cell-associated forms of human immunodeficiency virus and assessment of platelet function in vivo.
复制标题
补骨脂素介导的血小板浓缩物光净化：人类免疫缺陷病毒的无细胞和细胞相关形式的灭活以及体内血小板功能的评估。
DOI：
10.1046/j.1537-2995.1997.37997454013.x
复制
发表时间：
1997
期刊：
影响因子：
2.9
通讯作者：
Blajchman,MA
中科院分区：
文献类型：
--
作者：
Margolis-Nunno,H;Bardossy,L;Robinson,R;Ben-Hur,E;Horowitz,B;Blajchman,MA
研究人员正在研究用peptiens和宽带紫外线A（UVA）辐射处理血小板浓缩物（PC），以消除可能存在于捐献血液中的病原体。研究设计和方法将三种形式的人类免疫缺陷病毒（HIV）-无细胞的、活性复制的和潜伏感染的细胞系-加入到PC中，并用50微克/mL的4′-氨甲基-4，5 ′，8-三甲基补骨脂素（AMT）、0.35 mM芦丁、以及宽带和窄带UVA光（分别为320 - 400 nm和360-370 nm [UVA 1]）。在组织培养中评估加入的HIV的灭活;在血小板减少的兔子中评估血小板止血活性。在用30 J/cm 2的UVA 1光处理时，每种形式的HIV完全灭活（>或= 10（5）感染单位）。在处理试管中的2.5 mL PC或血袋中的完整PC单位（50 mL）时获得了相似的结果。潜伏感染的细胞系比无细胞HIV或活跃复制的HIV更敏感。经40 J/cm 2 UVA 1光处理的人血小板在处理后不久或储存5天后具有完全校正的出血时间，如在血小板减少兔中所评估的。血小板止血功能在UVA 1光为81 J/cm 2时开始下降，在UVA 1光为113 J/cm 2时消失。在相似的能量密度下，宽波段UVA光对血小板的伤害比UVA 1光更大。结论经AMT和UVA 1光处理后，PC可消除HIV传播，且血小板止血功能不降低。
BACKGROUNDTreatment of platelet concentrates (PCs) with psoralens and broad‐band ultraviolet A (UVA) radiation is being examined for the elimination of pathogens that might be present in donated blood. Previous studies have demonstrated the inactivation of cell‐free viruses and the maintenance of platelet integrity with common in vitro assays.STUDY DESIGN AND METHODSHuman immunodeficiency virus (HIV) in three forms‐cell‐free, activity replicating, and latently infected cell lines‐was added to PCs and treated with 50‐microgram per mL of 4′‐ aminomethyl‐4,5′,8‐trimethylpsoralen (AMT), 0.35 mM rutin, and broad‐ and narrow‐band UVA light (320‐400 nm and 360–370 nm [UVA1], respectively). The inactivation of added HIV was assessed in tissue culture; platelet hemostatic activity was assessed in thrombocytopenic rabbits.RESULTSEach form of HIV was inactivated completely (> or = 10(5) infectious units) on treatment with 30 J per cm2 of UVA1 light. Similar results were obtained on treatment of 2.5 mL of PCs in test tubes or intact PC units (50 mL) in blood bags. Latently infected cell lines were substantially more sensitive than cell‐free HIV or HIV that was actively replicating. Human platelets treated with 40 J per cm2 of UVA1 light had a fully corrected bleeding time shortly after treatment or after 5 days' storage, as assessed in thrombocytopenic rabbits. Platelet hemostatic function began to decrease with 81 J per cm2 of UVA1 light and was abolished with 113 J per cm2. At similar fluences, broad‐band UVA light was more injurious to platelets than was UVA1 light.CONCLUSIONHIV transmission might be eliminated by PCs after treatment with AMT and UVA1 light and without a reduction in platelet hemostatic function.