ABCC2 is a functional receptor of Bacillus thuringiensis Cry1Ca in Spodoptera litura.
ABCC2 is a functional receptor of Bacillus thuringiensis Cry1Ca in Spodoptera litura.
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DOI：
10.1016/j.ijbiomac.2021.11.174
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发表时间：
2021-11
影响因子：
8.2
通讯作者：
Xue Yao;Chen Liu;Yunpeng Duan;S. An;Jizhen Wei;G. Liang
中科院分区：
文献类型：
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作者：
Xue Yao;Chen Liu;Yunpeng Duan;S. An;Jizhen Wei;G. Liang
斜纹夜蛾（Spodoptera litura）是一种严重的多食性害虫，对大多数常用杀虫剂产生了抗药性，甚至对某些苏云金杆菌（Bacillus thuringiensis，Bt）毒素产生了抗药性。Cry 1Ca具有良好的杀虫活性。具有潜在应用价值的斜纹夜蛾。延缓害虫抗性的发展。然而，Cry 1Ca在斜纹夜蛾中的作用方式知之甚少。在此，Cry 1Ca结合蛋白被鉴定自S.使用下拉测定和液相色谱-串联质谱法（LC-MS/MS）测定lituraby。结果表明，Cry 1Ca可能与氨肽酶N（APN）、ATP结合盒亚家族C成员2（ABCC 2）、polycalin、actin和V型质子ATP酶亚基A结合。配体印迹和同源竞争实验证实ABCC 2片段与Cry 1Ca结合。Sf 9细胞中内源性SlABCC 2的过表达增加了Cry 1Ca的细胞毒性。相应地，S1 ABCC 2小干扰RNA（siRNA）在斜纹夜蛾中的功能分析的存活损失降低了Cry 1Ca对幼虫的毒性。这些结果表明，S. liturais是参与Cry 1Ca作用模式的功能性受体。
Spodoptera litura is a serious polyphagous pest in the whole world, which has developed resistance to most conventional insecticides and even someBacillus thuringiensis(Bt) toxins. Cry1Ca has excellent insecticide activity againstS. liturawith potential application to controlS. lituraand delay the development of insect resistance. However, the mode of action of Cry1Ca inS. liturais poorly understood. Here, Cry1Ca-binding proteins were identified fromS. lituraby using pull down assays and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The results indicated that aminopeptidase-N (APN), ATP binding cassette subfamily C member 2 (ABCC2), polycalin, actin and V-type proton ATPase subunit A may bind with Cry1Ca. Further study confirmed that ABCC2 fragment expressedin vitrocan bind to Cry1Ca as demonstrated by Ligand blot and homologous competition experiments. The over-expression of endogenousSlABCC2in Sf9 cells increased Cry1Ca cytotoxicity. Correspondingly, thevivoloss of function analyses bySlABCC2small interfering RNAs (siRNAs) inS. lituralarvae decreased the toxicity of Cry1Ca to larvae. Altogether, these results show that ABCC2 ofS. liturais a functional receptor that is involved in the action mode of Cry1Ca.