Establishment of 2.5D organoid culture model using 3D bladder cancer organoid culture
Establishment of 2.5D organoid culture model using 3D bladder cancer organoid culture
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DOI：
10.1038/s41598-020-66229-w
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发表时间：
2020-06-10
影响因子：
4.6
通讯作者：
Sasaki, Kazuaki
中科院分区：
文献类型：
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作者：
Abugomaa, Amira;Elbadawy, Mohamed;Sasaki, Kazuaki
三维（3D）类器官培养在癌症精准医学中具有广阔的前景。然而，基质胶和干细胞刺激补充剂是培养 3D 类器官细胞所必需的。与二维培养细胞相比，它需要花费大量资金并消耗更多的时间和精力。因此，需要建立更便宜且无基质胶的类器官培养物，能够保持部分3D类器官的特性。在之前的研究中，我们利用狗的尿液样本建立了狗膀胱癌（BC）3D类器官培养系统。在这里，我们使用 2.5 类器官培养基成功地从多种狗 BC 3D 类器官品系中分离出名为“2.5D 类器官”的细胞。 2.5D类器官的细胞增殖速度比亲代3D类器官细胞快。干细胞标志物的表达模式接近3D类器官。将2.5D类器官细胞注射到免疫缺陷小鼠体内形成肿瘤，并显示出与注射狗BC 3D类器官类似的尿路上皮癌的组织病理学特征。 2.5D 类器官对于抗癌药物治疗的敏感性与其亲本 3D 类器官相似。这些数据表明，我们建立的 2.5D 类器官培养方法可能会成为狗 BC 研究和治疗中合理且有用的工具，而不是 3D 类器官。
Three-dimensional (3D) organoid culture holds great promises in cancer precision medicine. However, Matrigel and stem cell-stimulating supplements are necessary for culturing 3D organoid cells. It costs a lot of money and consumes more time and effort compared with 2D cultured cells. Therefore, the establishment of cheaper and Matrigel-free organoid culture that can maintain the characteristics of a part of 3D organoids is demanded. In the previous study, we established a dog bladder cancer (BC) 3D organoid culture system by using their urine samples. Here, we successfully isolated cells named "2.5D organoid" from multiple strains of dog BC 3D organoids using 2.5 organoid media. The cell proliferation speed of 2.5D organoids was faster than parental 3D organoid cells. The expression pattern of stem cell markers was close to 3D organoids. Injection of 2.5D organoid cells into immunodeficient mice formed tumors and showed the histopathological characteristics of urothelial carcinoma similar to the injection of dog BC 3D organoids. The 2.5D organoids had a similar sensitivity profile for anti-cancer drug treatment to their parental 3D organoids. These data suggest that our established 2.5D organoid culture method might become a reasonable and useful tool instead of 3D organoids in dog BC research and therapy.