Bacterial partition complexes segregate within the volume of the nucleoid
Bacterial partition complexes segregate within the volume of the nucleoid
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DOI：
10.1038/ncomms12107
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发表时间：
2016-07-01
影响因子：
16.6
通讯作者：
Nollmann, Marcelo
中科院分区：
文献类型：
--
作者：
Le Gall, Antoine;Cattoni, Diego I.;Nollmann, Marcelo
精确和快速的DNA分离是遗传物质正确遗传所必需的。在大多数细菌和古细菌中，这一过程是由一个广泛保守的有丝分裂样装置来保证的，在该装置中，NTR（帕拉）取代了分区复合物。竞争的观察和模型意味着完全不同的3D定位模式的组件的分区机械隔离期间。在这里，我们使用超分辨率显微镜在3D中定位相对于细菌染色体的分离装置的每个组件。我们发现，Par蛋白位于类核体积内，并揭示适当的体积定位和隔离的分区复合物需要ATP酶和DNA结合活性的ParA。最后，我们发现，分区系统的不同组成部分的定位模式与密集的染色体区域高度相关。我们提出了一种新的机制，其中类核提供了一个支架，以指导适当的隔离分区复合物。
Precise and rapid DNA segregation is required for proper inheritance of genetic material. In most bacteria and archaea, this process is assured by a broadly conserved mitotic-like apparatus in which a NTPase (ParA) displaces the partition complex. Competing observations and models imply starkly different 3D localization patterns of the components of the partition machinery during segregation. Here we use super-resolution microscopies to localize in 3D each component of the segregation apparatus with respect to the bacterial chromosome. We show that Par proteins locate within the nucleoid volume and reveal that proper volumetric localization and segregation of partition complexes requires ATPase and DNA-binding activities of ParA. Finally, we find that the localization patterns of the different components of the partition system highly correlate with dense chromosomal regions. We propose a new mechanism in which the nucleoid provides a scaffold to guide the proper segregation of partition complexes.