Co-Delivery of Ciprofloxacin and Colistin in Liposomal Formulations with Enhanced In Vitro Antimicrobial Activities against Multidrug Resistant Pseudomonas aeruginosa.
Co-Delivery of Ciprofloxacin and Colistin in Liposomal Formulations with Enhanced In Vitro Antimicrobial Activities against Multidrug Resistant Pseudomonas aeruginosa.
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在脂质体制剂中共同递送环丙沙星和粘菌素，增强针对多药耐药铜绿假单胞菌的体外抗菌活性。
DOI：
10.1007/s11095-018-2464-8
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发表时间：
2018
影响因子：
3.7
通讯作者：
Zhou,QiTony
中科院分区：
文献类型：
--
作者：
Wang,Shaoning;Yu,Shihui;Lin,Yuwei;Zou,Peizhi;Chai,Guihong;Yu,HeidiH;Wickremasinghe,Hasini;Shetty,Nivedita;Ling,Junhong;Li,Jian;Zhou,QiTony
PurposeThis study aims to develop liposomal formulations containing synergistic antibiotics of colistin and ciprofloxacin for the treatment of infections caused by multidrug-resistantPseudomonas aeruginosa.MethodsColistin (Col) and ciprofloxacin (Cip) were co-encapsulated in anionic liposomes by ammonium sulfate gradient. Particle size, encapsulation efficiency,in vitrodrug release andin vitroantibiotic activities were evaluated.ResultsThe optimized liposomal formulation has uniform sizes of approximately 100 nm, with encapsulation efficiency of 67.0% (for colistin) and 85.2% (for ciprofloxacin). Incorporation of anionic lipid (DMPG) markedly increased encapsulation efficiency of colistin (from 5.4 to 67.0%); however, the encapsulation efficiency of ciprofloxacin was independent of DMPG ratio. Incorporation of colistin significantly accelerated the release of ciprofloxacin from the DMPG anionic liposomes.In vitrorelease of ciprofloxacin and colistin in the bovine serum for 2 h were above 70 and 50%. The cytotoxicity study using A549 cells showed the liposomal formulation is as non-toxic as the drug solutions. Liposomal formulations of combinations had enhancedin vitroantimicrobial activities against multidrug resistantP. aeruginosathan the monotherapies.ConclusionsLiposomal formulations of two synergistic antibiotics was promising against multidrug resistantP. aeruginosainfections.
