UPLC™:: An introduction and review
UPLC™:: An introduction and review
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DOI：
10.1081/jlc-200053046
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发表时间：
2005-01-01
影响因子：
1.3
通讯作者：
Swartz, ME
中科院分区：
文献类型：
--
作者：
Swartz, ME
超高效液相色谱（TM） （UPLC）利用了粒子化学性能、系统优化、检测器设计以及数据处理和控制方面的技术进步。使用低于2 μ m的颗粒和高线速度的流动相，以及在比HPLC更高的压力下工作的仪器，可以获得分辨率，灵敏度和分析速度的显着提高。这一分析分离科学的新类别保留了HPLC的实用性和原理，同时创造了色谱性能的阶梯功能改进。本文介绍了UPLC的理论，并对该领域的一些最新工作进行了总结。
Ultra performance liquid chromatography (TM) (UPLC) takes advantage of technological strides made in particle chemistry performance, system optimization, detector design, and data processing and control. Using sub-2 mu m particles and mobile phases at high linear velocities, and instrumentation that operates at higher pressures than those used in HPLC, dramatic increases in resolution, sensitivity, and speed of analysis can be obtained. This new category of analytical separation science retains the practicality and principles of HPLC while creating a step-function improvement in chromatographic performance.This review introduces the theory of UPLC, and summarizes some of the most recent work in the field.