Transcription of orthopoxvirus telomeres at late times during infection.
Transcription of orthopoxvirus telomeres at late times during infection.
复制标题
感染后期正痘病毒端粒的转录。
DOI：
10.1016/0042-6822(90)90187-v
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发表时间：
1990
期刊：
影响因子：
3.7
通讯作者：
Pickup,DJ
中科院分区：
文献类型：
--
作者：
Parsons,BL;Pickup,DJ
正痘病毒DNA的端粒主要由至少两组短的重复序列组成。虽然正痘病毒端粒的序列组成是高度保守的，但这些区域似乎不编码任何蛋白质。在感染后期，牛痘病毒的端粒被转录。在两组重复序列之间的区域中的启动子引导转录朝向病毒DNA的发夹环末端。该启动子类似于其他痘病毒晚期基因的启动子，并指导RNA的合成，其结构与晚期RNA典型的5′ poly（A）序列的存在一致。这些晚期转录本的长度表明，一些转录延伸通过发夹环区域。这可能发生在基因组为单体形式或基因组为DNA复制中间体的多联体形式时。端粒的晚期转录功能尚不清楚，但牛痘病毒、牛痘病毒和浣熊痘病毒端粒的类似转录表明，这种转录可能在病毒复制中起作用。
The telomeres of orthopoxvirus DNAs consist largely of short repeated sequences organized into at least two separate sets. Although the sequence composition of the orthopoxvirus telomeres is highly conserved, these regions do not appear to encode any proteins. At late times during infection, the telomeres of vaccinia virus are transcribed. A promoter in the region between the two sets of repeats directs transcription towards the hairpin-loop end of the viral DNA. This promoter resembles the promoters of other poxvirus late genes, and directs the synthesis of RNAs whose structure is consistent with the presence of 5′ poly(A) sequences typical of late RNAs. The lengths of these late transcripts suggest that some transcription extends through the hairpin-loop region. This might occur either when the genome is in a monomeric form or when the genome is in the concatemeric form of the DNA replication intermediate. The function of late transcription of the telomeres is unclear, but similar transcription of the telomeres of vaccinia virus, cowpox virus, and raccoonpox virus suggests that such transcription may have a role in viral replication.