Cell selective targeting of a simian virus 40 virus-like particle conjugated to epidermal growth factor
Cell selective targeting of a simian virus 40 virus-like particle conjugated to epidermal growth factor
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DOI：
10.1016/j.jbiotec.2011.06.030
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发表时间：
2011-09-10
影响因子：
4.1
通讯作者：
Handa, Hiroshi
中科院分区：
文献类型：
--
作者：
Kitai, Yuichi;Fukuda, Hajime;Handa, Hiroshi
猿猴病毒 40 (SV40) 病毒样颗粒 (VLP) 是用于基因传递的有效纳米载体。制备了与人表皮生长因子（hEGF）缀合的 VLP，并使用过表达 EGF 受体的人上皮癌 A431 细胞检查了 VLP 的细胞选择性。内吞效率由 hEGF 缀合的 VLP 中封装蛋白的 Gaussia 荧光素酶活性水平决定。 EGF 受体介导的 hEGF 结合的 VLP 的内吞作用显着增加，并通过使用封装在 hEGF 结合的 VLP 中的 mCherry 进行荧光成像来证实。这些结果表明，与特定配体缀合的 SV40 的 VLP 可用于细胞选择性基因递送。 (C) 2011 Elsevier B.V. 保留所有权利。
Simian virus 40 (SV40) virus-like particles (VLPs) are efficient nanocarriers for gene delivery. VLPs conjugated to human epidermal growth factor (hEGF) were prepared and the cell selectivity of the VLP was examined using human epithelial carcinoma A431 cells, which overexpress the EGF receptor. The endocytic efficiency was determined by the level of Gaussia luciferase activity from the encapsulated protein in hEGF-conjugated VLPs. EGF receptor-mediated endocytosis of hEGF-conjugated VLPs was significantly increased and was confirmed by fluorescence imaging using mCherry encapsulated in hEGF-conjugated VLPs. These results suggest that VLPs of SV40 conjugated to a specific ligand could be used for cell selective gene delivery. (C) 2011 Elsevier B.V. All rights reserved.