Uncoupling of hTREX demonstrates that UAP56 and hTHO-complex recruitment onto herpesvirus saimiri intronless transcripts is required for replication
Uncoupling of hTREX demonstrates that UAP56 and hTHO-complex recruitment onto herpesvirus saimiri intronless transcripts is required for replication
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DOI：
10.1099/vir.0.010124-0
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发表时间：
2009-06-01
影响因子：
3.8
通讯作者：
Whitehouse, Adrian
中科院分区：
文献类型：
--
作者：
Colgan, Kevin J.;Boyne, James R.;Whitehouse, Adrian
疱疹病毒 saimiri (HVS) ORF57 核胞质穿梭蛋白结合病毒 RNA 并与细胞核输出接头蛋白 Aly 相互作用，以进入 TAP 介导的核输出途径。这使得 HVS 无内含子 mRNA 能够有效地核输出。在此，我们扩展了这些研究，并证明 ORF57 将 hTREX 的几个成员，即 Aly、UAP56 和 hTHO 复合蛋白招募到病毒 mRNA 上，以组装具有输出能力的核糖核蛋白颗粒。此外，使用抑制 UAP56 和 hTHO 复合物与病毒无内含子 mRNA 关联的 Aly 反式显性形式，我们表明，有效的 HVS mRNA 核输出和复制需要完整的 hTREX 募集。
Herpesvirus saimiri (HVS) ORF57 nucleocytoplasmic shuttle protein binds viral RNA and interacts with the cellular nuclear export adaptor protein, Aly, to access the TAP-mediated nuclear export pathway. This enables the efficient nuclear export of HVS intronless mRNAs. Herein, we extend these studies and demonstrate that ORF57 recruits several members of hTREX, namely Aly, UAP56 and hTHO-complex proteins, onto the viral mRNAs to assemble an export-competent ribonucleoprotein particle. Moreover, using a transdominant form of Aly which inhibits UAP56 and hTHO-complex association with viral intronless mRNA, we show that complete hTREX recruitment is required for efficient HVS mRNA nuclear export and replication.