Identification of cross-reactive promastigote cell surface antigens of some leishmanial stocks by 125I labeling and immunoprecipitation
Identification of cross-reactive promastigote cell surface antigens of some leishmanial stocks by 125I labeling and immunoprecipitation
复制标题
通过 125I 标记和免疫沉淀鉴定某些利什曼原虫种的交叉反应前鞭毛体细胞表面抗原
DOI：
10.1128/iai.43.2.637-643.1984
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发表时间：
1984
影响因子：
3.1
通讯作者：
D. Dwyer
中科院分区：
文献类型：
--
作者：
P. Gardiner;C. Jaffe;D. Dwyer
用125I放射性标记了几种利什曼原虫前鞭毛体的外向表面膜成分。细胞表面标记的蛋白质和十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法分离的蛋白质的放射自显影显示出在6,000至240,000分子量范围内的独特条带模式。用抗杜氏利什曼原虫膜血清对标记的前鞭毛体的洗涤剂提取物进行免疫沉淀，结果表明每个标记的前鞭毛体都含有一些抗原性交叉反应决定簇。这些决定因素的电泳谱既用于区分寄生虫种群(通过独特的、物种特定的模式)，也用于指示按其他生化标准被认为不同的种群的抗原相似性。在两种新大陆利什曼原虫中，至少有12种交叉反应细胞表面抗原被多价抗L识别。多诺瓦尼血清，提示这些常见的利什曼原虫抗原可能解释了各种利什曼原虫之间的血清学交叉反应。在所有被测试的原料中，鉴定出一种碘化蛋白质，在还原条件下相对分子质量为65,000，但在非还原条件下十二烷基硫酸钠-聚丙烯酰胺凝胶的相对流动性增加。通过使用单抗，还显示了几个种群共有的抗原的独特模式。
Externally oriented surface membrane constituents of promastigotes from several Leishmania species were radiolabeled with 125I. Autoradiographs of cell surface-labeled and sodium dodecyl sulfate-polyacrylamide gel electrophoresis-separated proteins of the stocks revealed distinctive patterns of bands in the molecular weight range of 6,000 to 240,000. Immunoprecipitation of detergent extracts of the labeled promastigote stocks with anti-Leishmania donovani membrane serum demonstrated that each of the stocks contained some antigenically cross-reactive determinants. The electrophoretic patterns of these determinants serve both to distinguish the parasite stocks (by unique, species-specific patterns) and to indicate antigenic similarities in stocks thought to be different by other biochemical criteria. At least 12 cross-reactive cell surface antigens in two New World leishmanias are recognized by polyvalent anti-L. donovani serum, suggesting that these common leishmanial antigens may account for the documented serological cross-reactivities among various Leishmania species. In all stocks tested, an iodinated protein was identified which had a relative molecular weight of 65,000 under reducing conditions but which demonstrated an increase in relative mobility in sodium dodecyl sulfate-polyacrylamide gels under nonreducing conditions. Distinctive patterns of the antigens common to the several stocks were also demonstrated with the use of monoclonal antibodies.