Genetic and physiological diversity of phylogenetically and geographically distinct groups of Arthrobacter isolated from lead-zinc mine tailings.
Genetic and physiological diversity of phylogenetically and geographically distinct groups of Arthrobacter isolated from lead-zinc mine tailings.
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DOI：
10.1016/j.femsec.2004.04.009
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发表时间：
2004-08
影响因子：
4.2
通讯作者：
Zhang Hanbo;Changqun Duan;Qiyong Shao;Weimin Ren;S. Tao;Lizhong Cheng;Zhiwei Zhao;Hussein bin.
中科院分区：
文献类型：
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作者：
Zhang Hanbo;Changqun Duan;Qiyong Shao;Weimin Ren;S. Tao;Lizhong Cheng;Zhiwei Zhao;Hussein bin.
对从3个尾矿堆中分离的60株细菌进行16SrRNA基因部分序列分析，确定其系统发育位置。这些菌株被分为三个不同的节肢动物群，可能代表了几个未描述的物种。根据对5种重金属（Zn、Pb、Cd、Cu和Co）和4种抗生素（Kan、Rif、Str和Ketamine）的抗性，以及对49种碳源利用的差异，评价了不同地理种群的生理差异。随机扩增多态性DNA（RAPD）分析表明，遗传分化。这些生物学参数显着不同的三个群体之间。值得注意的是，可检测的差异之间也观察到三个地理上不同的细分与类似的分类位置。这些结果表明，尾矿是一个理想的网站，调查极端金属污染的自然细菌种群的分化。此外，这些环境似乎窝藏许多以前没有特征的细菌物种，这是潜在的重要候选人，由于其显着的耐多种金属的生物修复应用。
The phylogenetic positions of 60 bacterial strains isolated from three tailing piles were determined by analyzing their partial 16S rRNA gene sequences. These strains were divided into three phylogenetically distinct groups ofArthrobacterand likely represented several non-described species. The physiological diversities of these phylogenetically and geographically distinct populations were evaluated based on their resistance to five heavy metals (Zn, Pb, Cd, Cu and Co) and four antibiotics (Kan, Rif, Str and Amp), and differences in utilization of 49 carbon sources. Genetic differentiations were demonstrated with randomly amplified polymorphic DNA (RAPD) analysis. These biological parameters were significantly different among three phylogenetically distinct groups. Notably, detectable differences were also observed among three geographically distinct subdivisions with similar taxonomic position. These results indicate that mine tailings are an ideal site for investigating the differentiation of natural bacterial populations in response to extreme metal contamination. Additionally, these environments appear to harbor many previously not characterized bacterial species, which are potentially important candidates for application in bioremediation due to their remarkable resistance to multiple metals.