Refrigerated storage and cryopreservation of sperm of red drum, Sciaenops ocellatus L.
Refrigerated storage and cryopreservation of sperm of red drum, Sciaenops ocellatus L.
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DOI：
10.1111/j.1365-2109.1998.tb01131.x
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发表时间：
1998-04-01
影响因子：
2
通讯作者：
Thomas, RG
中科院分区：
文献类型：
--
作者：
Wayman, WR;Tiersch, TR;Thomas, RG
为了辅助石斑鱼的人工产卵，本研究建立了红鱼精液的采集、处理和冷冻保存技术。精液是通过切除和切片精巢，并加入200-400mOsm kg(-1)的Hanks平衡盐溶液或NaCl液作为延伸剂收集的。用800mOsm kg(-1)人工海水(ASW)激活精子，以表征精子的活力。精子在8+/-1 S(平均值+/-SD)内达到最大活力，并保持在最大活力33+/-4 S。为了确定引起活力的渗透压范围，精子暴露在ASW(8-800mOsm kg(-1))的不同渗透压下，阈值激活(类似于10%活力)发生在351+/-4mOsm kg(-1)，而完全激活发生在539+/-2mOsm(-1)。保存在200mOsm kg(-1)下的精子可保持活力长达13天。在200mOsm kg(-1)的HBSS中，二甲基亚砜(DMSO)作为冷冻保护剂，其浓度范围为7.5%~15%(v：v)。不同浓度的DMSO冷冻保存的精子解冻后活力无显著差异。在21摄氏度的台面上解冻的精子在解冻后的活力低于在10、20、30、40、50或60摄氏度的水浴中解冻的精子。
To aid in artificial spawning of sciaenid fishes, the present authors developed techniques to collect, handle and cryopreserve sperm from red drum, Sciaenops ocellatus L. Sperm were collected by removing and slicing the testis, and adding Hanks' balanced salt solution (HBSS) or NaC1 solution teach at 200-400 mOsm kg(-1)) as an extender. Sperm were activated with 800 mOsm kg(-1) artificial sea water (ASW) to characterize motility. Sperm reached maximum motility highest percentage motility observed for that sample) within 8 +/- 1 s (mean +/- SD) and remained at maximum motility for 33 +/- 4 s. Sperm were exposed to graded osmotic pressures of ASW (8-800 mOsm kg(-1)) to determine the range of osmolalities that elicited motility, Threshold activation (defined as similar to 10% motility) occurred at 351 +/- 4 mOsm kg(-1) and complete activation occurred at 539 +/- 2 mOsm kg(-1). Sperm stored at 200 mOsm kg(-1) retained motility for up to 13 days. Dimethyl sulphoxide (DMSO) was used as a cryoprotectant at concentrations ranging from 7.5% to 15% (v:v) in HBSS (200 mOsm kg(-1)). There were no significant differences among postthaw motilities of sperm cryopreserved at any concentration of DMSO. Sperm thawed on the benchtop at 21 degrees C had lower post-thaw motility than did sperm thawed at 10, 20, 30, 40, 50 or 60 degrees C in a water bath.