Minocycline hydrochloride loaded on titanium by graphene oxide: An excellent antibacterial platform with the synergistic effect of contact-killing and release-killing
Minocycline hydrochloride loaded on titanium by graphene oxide: An excellent antibacterial platform with the synergistic effect of contact-killing and release-killing
复制标题
氧化石墨烯负载盐酸米诺环素：具有接触杀灭和释放杀灭协同作用的优异抗菌平台
DOI：
10.1039/c7bm00931c
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发表时间：
2018
影响因子：
6.6
通讯作者：
Xuanyong Liu
中科院分区：
文献类型：
--
作者：
Wenhao Qian;Jiajun Qiu;Jiansheng Su;Xuanyong Liu
钛及其合金具有优良的性能，在骨科和牙科植入物中得到了广泛的应用。然而，由于植入物相关的细菌感染，植入物失效仍然发生。因此，对钛及其合金进行适当的表面改性是十分必要的。在本工作中，商业纯钛板与氧化石墨烯（GO）的修饰，用于负载盐酸米诺环素。革兰氏阳性金黄色葡萄球菌（S.金黄色葡萄球菌）和变形链球菌（S.变形杆菌）和革兰氏阴性大肠杆菌（E.大肠杆菌（coli）的抑菌活性。应用人牙龈成纤维细胞（HGF）来评估各种样品的细胞相容性。为了研究细菌存在下细胞的粘附和细胞表面覆盖，将HGF细胞与S。金黄色葡萄球菌。结果表明，GO修饰的钛表面对直接接触GO的细菌有抑制作用，而对非直接接触GO的细菌无影响。盐酸米诺环素在GO修饰的钛表面（M@GO-Ti）表现出缓释行为，并表现出良好的抗菌活性，GO和盐酸米诺环素分别具有接触杀伤和释放杀伤的协同效应。在与S.在所有样品中，M@GO-Ti上的HGF细胞表现出最好的细胞活力和细胞表面覆盖。
Titanium and its alloys have been widely used in orthopedic and dental implants because of their excellent properties. However, implant failures still occur due to implant-associated bacterial infections. Therefore, proper surface modification of titanium and its alloys is necessary. In this work, commercial pure titanium plates were modified with graphene oxide (GO) which was used to load minocycline hydrochloride. Gram-positive Staphylococcus aureus (S. aureus) and Streptococcus mutans (S. mutans) and Gram-negative Escherichia coli (E. coli) were used to investigate the antibacterial activity of the samples. Human gingival fibroblast (HGF) cells were applied to assess the cytocompatibility of the various samples. To investigate cell adhesion and cell surface coverage in the presence of bacteria, the coculture of HGF cells and S. aureus was performed. The results indicated that the GO-modified titanium surface could inhibit the growth of the bacteria which had direct contact with GO, while it could not affect the bacteria without direct contact of GO. Minocycline hydrochloride on the GO-modified titanium surface (M@GO-Ti) showed a slow release behavior and exhibited excellent antibacterial activity with the synergistic effect of contact-killing and release-killing by GO and minocycline hydrochloride, respectively. In the coculture process with the presence of S. aureus, HGF cells on M@GO-Ti demonstrated the best cell viability and cell surface coverage among all the samples.