ANALYSIS OF A HYPERVARIABLE REGION IN THE 3' NONCODING END OF THE INFECTIOUS-BRONCHITIS VIRUS GENOME
ANALYSIS OF A HYPERVARIABLE REGION IN THE 3' NONCODING END OF THE INFECTIOUS-BRONCHITIS VIRUS GENOME
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DOI：
10.1016/0168-1702(93)90086-3
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发表时间：
1993-04-01
期刊：
影响因子：
5
通讯作者：
COLLISSON, EW
中科院分区：
文献类型：
--
作者：
WILLIAMS, AK;WANG, L;COLLISSON, EW
先前对传染性支气管炎病毒 (IBV) cDNA 的研究已在美国原型毒株 (Beaudette) 和日本毒株 (KB8523) 的 3' 非编码末端鉴定出约 184 个碱基的区域，该区域在抗原性密切相关的美国毒株马萨诸塞州 (Mass) 41 中不存在（Boursnell 等人，1985 年；Sutou 等人，1988 年）。为了研究该区域的起源和功能及其在自然界中的发生，测定了另外三种IBV菌株Gray、Arkansas (Ark) 99和Holland (Holl) 52的3'非编码区的cDNA序列，并与Beaudette、KB8523和Mass41菌株的序列进行比较。 Mass41 菌株的 3' 非编码区不仅不存在这种富含 U 的序列，而且它也具有高度可变性，特别是与该序列下游高度保守的 3' 非编码区相比。对该高变区 (HVR) 邻近序列的计算机分析表明，IBV 基因组的 3' 端在该区域的下游高度保守，相似性为 94.3% 至 97.8%。然而，HVR 的相似度从 Holl52 和 Ark99 之间的 53.2% 到 Beaudette 和 Gray 之间的 92.8% 不等。侧翼序列不仅是保守的，而且HVR上游和下游的这些序列也形成镜像。
Previous studies on infectious bronchitis virus (IBV) cDNA have identified a region of about 184 bases in the 3' non-coding terminus of both the U.S. prototype strain (Beaudette) and a Japanese strain (KB8523), that was not present in an antigenically closely related U.S. strain, Massachusetts (Mass) 41 (Boursnell et al., 1985; Sutou et al., 1988). In order to investigate the origin and function of this region and its occurrence in nature, the cDNA sequences of the 3' non-coding regions of three additional strains of IBV, Gray, Arkansas (Ark) 99 and Holland (Holl) 52, were determined and compared to the sequences of the Beaudette, KB8523 and Mass41 strains. Not only was this U-rich sequence absent from the 3' non-coding region of the Mass41 strain, it was also highly variable, especially in comparison to the highly conserved 3' non coding region downstream of this sequence. Computer analyses of the sequences adjacent to this hypervariable region (HVR) showed that the 3' end of the IBV genome was highly conserved downstream of this region, with 94.3 to 97.8% similarity. However, the similarities for the HVR ranged from 53.2% between Holl52 and Ark99, to 92.8% between Beaudette and Gray. The flanking sequences were not only conserved but these sequences upstream and downstream of the HVR also formed mirrored images.