ANALYSIS OF SOLUTION EFFECTS INJURY EQUATIONS FOR CALCULATING PHASE-DIAGRAM INFORMATION FOR THE TERNARY-SYSTEMS NACL-DIMETHYLSULFOXIDE-WATER AND NACL-GLYCEROL-WATER
ANALYSIS OF SOLUTION EFFECTS INJURY EQUATIONS FOR CALCULATING PHASE-DIAGRAM INFORMATION FOR THE TERNARY-SYSTEMS NACL-DIMETHYLSULFOXIDE-WATER AND NACL-GLYCEROL-WATER
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DOI：
10.1016/s0006-3495(80)85019-3
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发表时间：
1980-01-01
影响因子：
3.4
通讯作者：
FAHY, GM
中科院分区：
文献类型：
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作者：
FAHY, GM
缓慢冷冻的细胞据说会受到溶液效应的伤害。对溶液效应损伤机制的理解依赖于对冷冻过程中细胞外液的成分变化的理解。为了便于分析缓慢降温过程中的冻害机理，建立了可以描述氯化钠-二甲基亚砜-水三元体系和氯化钠-甘油-水三元体系组成变化的经验方程。这些方程描述了相图中仅由于冰的沉淀而引起成分变化的区域。目前的相图方程可以重新排列，以给出组成变量的表达式，如水含量、盐浓度、溶液的未冻结分数等，这些变量可用于分析溶液组成与溶液伤害效应之间的关系。
Slowly frozen cells are said to be subject to solution effects injury. An understanding of the mechanism of solution effects injury depends upon an understanding of the compositional changes brought about in the extracellular solution during the freezing process. To facilitate analysis of the mechanisms of freezing injury during slow cooling, empirical equations were developed which permit a description of these changes in composition for the NaCl-dimethylsulfoxide-water ternary system, and for the NaCl-glycerol-water ternary system. The equations describe the region of the phase diagram in which compositional changes are brought about only as a result of the precipitation of ice. The present phase diagram equations may be rearranged to give expressions for composition variables such as water content, salt concentration, unfrozen fraction of the solution, etc., which may be employed in the analysis of the relationship between solution composition and solution effects injury.