Immune responses of Holstein and Norwegian Red x Holstein calves on Canadian dairy farms
Immune responses of Holstein and Norwegian Red x Holstein calves on Canadian dairy farms
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DOI：
10.3168/jds.2008-1300
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发表时间：
2009-02-01
影响因子：
3.5
通讯作者：
Mallard, B. A.
中科院分区：
文献类型：
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作者：
Begley, N.;Buckley, F.;Mallard, B. A.
本研究的目的是比较13个商业加拿大奶牛场荷斯坦和挪威红花荷斯坦牛犊的免疫应答。收集了135头2~6个月龄的小母牛、68头荷斯坦公牛和67头挪威红×荷斯坦公牛的数据。用蛋清溶菌酶免疫犊牛，诱导抗体介导的免疫应答。以白念珠菌作为细胞免疫反应的体内指标，以迟发型超敏反应为指标。用酶联免疫吸附试验检测蛋清溶菌酶(Ig G、Ig G1和Ig G2)的抗体反应。这两个品种的小牛都产生了显著的一次和二次抗体介导的免疫反应，以及迟发性超敏反应。与荷斯坦牛相比，挪威红×荷斯坦牛产生了更强的初次免疫应答(d14，和d14减去d0)。在二次反应、抗蛋清溶菌酶同型(IgG1或IgG2)产生和IgG1/IgG2比值方面，品种间差异不显著。品种对迟发型超敏反应无影响。尽管如此，在两个品种群中都可以识别出高和低免疫反应者，但在不同品种群之间观察到的抗体介导的免疫反应或细胞介导的免疫反应的高反应者和低反应者的比例没有差异。
The objective of this study was to compare the immune response of Holstein and Norwegian Red x Holstein calves on 13 commercial Canadian dairy farms. Data were collected on 135 calves, 68 Holstein and 67 Norwegian Red x Holstein calves aged between 2 and 6 mo. The calves were immunized with hen egg white lysozyme to induce antibody-mediated immune response. Candida albicans was used as an in vivo indicator of cell-mediated immune response, with delayed-type hypersensitivity used as the indicator. Antibody response to hen egg white lysozyme (IgG, IgG1, and IgG2) was measured by ELISA. Calves of both breed groups produced a significant primary and secondary antibody-mediated immune response, as well as a delayed-type hypersensitivity reaction. The Norwegian Red x Holstein produced a greater primary IgG antibody-mediated immune response (d 14, and d 14 minus d 0) when compared with the Holstein. No differences were observed between the breeds for secondary response or anti-hen egg white lysozyme isotype (IgG1 or IgG2) production or the ratio of IgG1: IgG2. There was no effect of breed on delayed-type hypersensitivity. Nonetheless, high and low immune responders could be identified in both breed groups, but with no difference in the proportion of high and low responders observed for either antibody-mediated immune response or cell-mediated immune response between breed groups.