Oral and pharyngeal epithelial keratinocyte culture.
Oral and pharyngeal epithelial keratinocyte culture.
复制标题
DOI：
10.1007/978-1-62703-125-7_5
复制
发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Gutkind, J Silvio
中科院分区：
文献类型：
--
作者：
Leelahavanichkul, Kantima;Gutkind, J Silvio
原代人口腔上皮细胞是容易获得的，最近已被用于组织工程。这些细胞目前被广泛用于多种研究工作，从口腔生物学、粘膜免疫和致癌作用的研究到干细胞生物学和组织工程。本章介绍了一步一步的协议，成功的分离和培养人口腔上皮细胞和成纤维细胞，和技术，他们在二维和三维培养系统中的使用。所描述的方法将能够在体外产生类似于上皮样结构的重构组织，其可以概括体内口腔上皮的一些关键特征。
Primary human oral epithelial cells are readily available and have been recently employed for tissue engineering. These cells are currently being widely utilized in multiple research efforts, ranging from the study of oral biology, mucosal immunity, and carcinogenesis to stem cell biology and tissue engineering. This chapter describes step-by-step protocols for the successful isolation and culture of human oral epithelial cells and fibroblasts, and techniques for their use in two-dimensional and three-dimensional culture systems. The described methods will enable to generate reconstituted tissues that resemble epithelial like structures in vitro, which can recapitulate some of the key features of the oral epithelium in vivo.