THE ABA-1 ALLERGEN OF THE NEMATODE ASCARIS-SUUM - EPITOPE STABILITY, MASS-SPECTROMETRY, AND N-TERMINAL SEQUENCE COMPARISON WITH ITS HOMOLOG IN TOXOCARA-CANIS
THE ABA-1 ALLERGEN OF THE NEMATODE ASCARIS-SUUM - EPITOPE STABILITY, MASS-SPECTROMETRY, AND N-TERMINAL SEQUENCE COMPARISON WITH ITS HOMOLOG IN TOXOCARA-CANIS
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DOI：
10.1111/j.1365-2249.1993.tb05958.x
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发表时间：
1993-04-01
影响因子：
4.6
通讯作者：
KENNEDY, MW
中科院分区：
文献类型：
--
作者：
CHRISTIE, JF;DUNBAR, B;KENNEDY, MW
阿坝-1是蛔虫属线虫寄生虫的主要过敏原，蛔虫属线虫寄生虫包括人和猪的大蛔虫，A. lumbricoides和A.分别为。过敏原是从A.免疫亲和层析用于免疫化学检查。IgE抗体库在感染的啮齿动物中处于MHC控制之下，并且IgE结合表位对热或高碘酸盐处理以及硝酸纤维素上的电印迹是稳健的。这意味着IgE表位包含一级肽序列或异常稳定的二级或三级结构。阿坝-1的分子量存在争议，但质谱分析表明，有5个类似大小的组分，主要种类为14643 -2 +/-1.4D。最后，阿坝-1和TBA-1（在犬线虫感染人类，犬弓蛔虫的同源物）的N-末端序列分析显示了高度的相似性，我们以前的证据表明，阿坝-1同源物是广泛存在于人体蛔虫寄生虫。因此，阿坝-1及其同源物可能对许多线虫寄生虫感染的免疫病理学很重要，宿主的遗传组成也会对免疫病理学产生影响。
ABA-1 is a major allergen of nematode parasites of the genus Ascaris which includes the large roundworms of humans and pigs, A. lumbricoides and A. suum, respectively. The allergen was purified from A. suum by immunoaffinity chromatography for immunochemical examination. The IgE antibody repertoire is under MHC control in infected rodents and the IgE-binding epitopes were robust to treatment with heat or periodate, and electroblotting on nitrocellulose. This implies that the IgE epitopes comprise primary peptide sequence or an unusually stable secondary or tertiary structure. The molecular mass of ABA-1 is controversial, but mass spectrometry analysis indicated that there were five components of similar size, with the major species being 14 643-2 +/- 1.4 D. Finally, N-terminal sequence analysis of ABA-1 and TBA-1 (the homologue in the canine nematode infective to humans, Toxocara canis) revealed a high degree of similarity, and we have previous evidence that ABA-1 homologues are widespread amongst ascaridid parasites of humans. ABA-1 and its homologues might, therefore, be important to the immunopathology of many infections with nematode parasites, upon which the genetic constitution of the hosts will also have a bearing.