PASSAGE OF AN INTEGRAL MEMBRANE-PROTEIN, THE VESICULAR STOMATITIS-VIRUS GLYCOPROTEIN, THROUGH THE GOLGI-APPARATUS EN ROUTE TO THE PLASMA-MEMBRANE
PASSAGE OF AN INTEGRAL MEMBRANE-PROTEIN, THE VESICULAR STOMATITIS-VIRUS GLYCOPROTEIN, THROUGH THE GOLGI-APPARATUS EN ROUTE TO THE PLASMA-MEMBRANE
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DOI：
10.1073/pnas.78.3.1746
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
SINGER, SJ
中科院分区：
文献类型：
--
作者：
BERGMANN, JE;TOKUYASU, KT;SINGER, SJ
本文用间接免疫荧光法和免疫电镜法对水泡性口炎病毒（VSV）跨膜糖蛋白的细胞内生物合成途径进行了原位研究。通过使用温度敏感的病毒突变体奥赛-45和温度下移方案使糖蛋白的运输同步。在粗面内质网、高尔基体和质膜中依次出现糖蛋白。该系统的进一步研究的潜力进行了讨论。
The intracellular pathway of biogenesis of the vesicular stomatitis virus transmembrane glycoprotein was investigated in situ by using indirect immunofluorescence of whole infected Chinese hamster ovary cells, and immunoelectron microscopy of ultrathin frozen sections of infected cells. Transport of the glycoprotein was synchronized by using the temperature-sensitive virus mutant, Orsay-45, and a temperature shift-down protocol. Sequential appearance of the glycoprotein in the rough endoplasmic reticulum, Golgi apparatus and plasmalemma was demonstrated. The potential of this system for further studies is discussed.