An experimental model of human body louse infection with Rickettsia prowazekii
An experimental model of human body louse infection with Rickettsia prowazekii
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DOI：
10.1086/345373
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发表时间：
2002-12-01
影响因子：
6.4
通讯作者：
Raoult, D
中科院分区：
文献类型：
--
作者：
Houhamdi, L;Fournier, PE;Raoult, D
普氏立克次体是通过体虱传播给人类的。本文报道了一种新的实验体虱感染模型。这里报道了prowazekii。800只人虱通过喂养一只兔子而感染，兔子通过注射2 × 10（6）个噬斑形成单位的R. prowazekii。该细菌侵入胃细胞并在粪便中释放，感染后5天在粪便中检测到。在感染后第6天，由于血淋巴中的细胞破裂和红细胞扩散，虱变成鲜红色，并在4 h内死亡。与未感染的对照虱相比，感染虱的寿命缩短了20-23天。感染的虱子不传播R。普瓦齐克传给后代。通过细胞培养，从排出后10天的粪便样品中培养立克次体。在喂虱期间给予兔强力霉素不能治愈来自R.普氏感染
Rickettsia prowazekii is transmitted to humans by the body louse. A new experimental model of body louse infection with R. prowazekii is reported here. Eight hundred human lice were infected by feeding on a rabbit that was made bacteremic by injecting 2 x 10(6) plaque-forming units of R. prowazekii. The bacterium invaded the stomach cells and was released in feces, in which it was detected 5 days after infection. At day 6 after infection, as a result of the cell burst and the spread of erythrocytes in the hemolymph, the louse became bright red and died within 4 h. The life span of infected lice was shortened by 20-23 days, compared with that of uninfected control lice. Infected lice did not transmit R. prowazekii to their progeny. Through cell culture, rickettsiae were cultivated from fecal samples up to 10 days after their emission. The administration of doxycycline to the rabbit during louse feeding did not cure lice from R. prowazekii infection.